Molecular conformations of araneus ventricosus spider ' s dragline silk in the spinning process 大腹园蛛牵引丝在纺丝过程中的分子构象
2.
Its repetitive motif mainly is ( ga ) nam ( ga ) n and the homology with araneus diadematus fibroin - 1 mrna ( adf - l ) is 69 . 3 % that is the maximum , too . avgl that is the no - silk protein gene was got 与现有已知的蛛丝蛋白基因中十字园蛛( araneusdiadematus )丝蛋白基因adf1有最高的同源性69 . 3 。
3.
Construction of araneus ventricousus ampullate gland cdna library and the finds of two new spider silk genes are the bases of supplying the background of araneus ventricousus silk genes and further researching in the spider silk proteins 大腹园蛛主壶腹腺cdna文库的构建和两个蛛丝蛋白新基因的克隆，为提供大腹园蛛蛛丝蛋白基因背景和进一步研究蛛丝蛋白奠定了基础。
4.
The cdna library of major ampullate glands of araneus ventricousus was prepared successfully for the first time by using puc18 vector in the world . two genes encoding spider silk protein and one gene encoding no - silk protein were cloned by means of randomly picking colony ( bird gun ) 本研究在国内外首次构建了大腹园蛛( araneusventricosus )主壶腹腺( majorampullategland ) cdna文库，并以鸟枪法从中筛选出两个蛛丝蛋白和一个非丝蛋白cdna新基因。
5.
Firstly , the major ampullate glands were got from araneus ventricousus . total rna and mrna of ampullate glands were isolated and purified . double strands cdnas were synthesized in the assistance of amv - rt and e . coli dna polymerase i etc . by reverse transcription and replacement synthesis 首先剥离大腹园蛛主壶腹腺，提取总rna和分离纯化mrna ，反转录合成cdna ，凝胶层析除去小于400bp片段，并在双链cdna两端引入ecor的衔接头，对其进行磷酸化处理。
