| 1. | Independent clones was constructed and affinity panning of anti - n - peptide antibody fragments was carried out
的小鼠噬菌体抗体库，并从中淘选出对n -肽有结合活性的单链抗体。 |
| 2. | The phagemid particles displaying functional scfvs were rescued by reinfection of helper phage m13ko7 , thus a murine antibody library was obtained
经辅助噬菌体m13ko7超感染回收全部重组噬菌体，此即噬菌体抗体库。 |
| 3. | The murine phage antibody library was amplified and then panned by human chorionic gonadotropin for four rounds . the last round enriched phage clones were used to reinfect
由鼠源噬菌体抗体库淘选到展示有人绒毛膜促性腺激素单链抗体的噬菌体克隆。 |
| 4. | In the present paper the application of dna library , phage display random peptide library , phage antibody library , etc . in the research of serodiagnostic reagents and their special values were reviewed
本文综述了基因文库、噬菌体展示肽库及噬菌体抗体库技术在血清学诊断试剂研制中的应用及其各自的优点。 |
| 5. | Amplified in e . coli xi - blue , the eluted phage in the third rounds was poured onto lb / iptg / xgal plate . we selected randomly 18 clones and amplified them , then confirmed positive clones by elisa
经双抗体(流感病毒的多抗和辣根过氧化物酶标记m13噬菌体抗体)夹心elisa鉴定的阳性克隆有12个，分别将其纯化、并进行dna序列分析。 |
| 6. | Pcr detection found there were 1 . 2x104 tfu in the library . one of colonies of scfv antibody , which binds to bull sperm , was selected and sequenced . sequence analysis indicates that the scfv colony resembles the mouse antibody
用phage - elisa从文库中筛选并鉴定出一个针对牛精子的阳性克隆，序列分析发现，该单链抗体克隆的序列符合小鼠抗体可变区的一般特征，这说明构建的针对牛精子的噬菌体抗体文库是成功的。 |
| 7. | The patterns of sds - page indicated more than 30 % of recombinant proteins could be obtained from the extract of e . coli bl21 . furthermore , library of recombinant phage antibodies was constructed from total rna isolated from spleen of the female balb / c mice immunized by bull sperm
首先用牛精子免疫雌性四周龄balb c小鼠，从其脾脏组织分离总rna ，应用重组噬菌体抗体库技术，构建了一个针对牛精子的噬菌体抗体文库。 |
| 8. | The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis
取阳性重组噬菌体抗体克隆株pcsa1 ， pcr扩增其scfv基因，筛选重组子进行序列测定，发现其序列符合小鼠抗体基因的一般特征，并且与几株抗磷酸胆碱的抗体重链和轻链可变区序列的同源性达80以上；推测pcsa1scfv针对的抗原是磷酸胆碱类物质。 |
