| 1. | The y - a489 - plex revealed stability , robustness and sensitivity in validation experiments . there was no interference of female for y - a489 - plex typing in mixture samples
结论本研究在国内外第一次将加尾序列引物设计策略引入y一str复合扩增体系。 |
| 2. | The analysis of population samples showed that 37 haplotypes were found from 100 unrelated males for the four y - str loci . the haplotype diversity was 0 . 9628 and standard error was 0 . 00429
加尾引物复合扩增策略还有很大的潜力，为大规模y一str复合扩增的研究提供了一个行之有效的方法。 |
| 3. | A careful comparison about the two ghrs was carried out as follows : scghr : the scghr cdna consists of 1958bp whose orf encodes a 602 aa protein , with a polyadnenylation signal and a ply ( a ) tail
南方鲇ghr ： scghrcdna全长1958bp ，包括一个编码602个氨基酸残基蛋白质的开放阅读框，一个加尾信号( aataa )和一个poly ( a )的尾巴。 |
| 4. | A late baculoviral transcription initiation motif ataag was found 65 nt upstream of the putative translational start site and a polyadenylation signal aataaa was identified 14 nt downstream of the taa stop codon
在其起始密码子上游- 65nt发现杆状病毒晚期启动子转录起始信号ataag ，在其终止密码子下游- 14nt发现polya加尾信号aataaa 。 |
| 5. | The tk gene contained an open reading frame of 957 bp encoding a 318 aa protein . upstream of the tk orf , three putative gc boxes are located at positions - 22 , - 166 and - 199 . a potential poly a signal begins 110 nucleotides downstream from the termination codon at position 1306
在tkorf上游- 22 、 - 166 、 - 199位存在3个gc框样序列，在终止密码子下游第110个核苷酸处的1306位存在有多聚腺苷加尾信号。 |
| 6. | Trptl cdna is 963bp in length containing an orf encoding 253aa with the molecular mass of 27 . 8kda and the pi of 10 . 13 . trptl is mapped to i1q12 . 3 spanning approximately 2 . 5kb on the chromosome . it consists of 8 exons and 7 introns , with the exception of the boundary between the 3 ' - end of first exon and the 5 ' - end of first intron , all are consistent with the gt - ag rule
主要结果如下： trpt1全长cdna序列共963bp ，含有一个编码253aa的开放阅读框，其5 ’ -端和3 ’ -端分别含有一个处于同一读框的终止密码和poly ( a )加尾信号，该读框编码蛋白的预测分子质量为27 . 8kda ，等电点为10 . 13 。 |
| 7. | A series of validation experiments and genetic studies should be performed for the y - str multiplex system according to the suggestion of the technical work group dna analysis methods ( twgdam ) . method we selected four y - str loci , dys434 , dys438 , dys439 , a10 ( y - gata - a10 ) and designed two set of tailed primers to improve the efficiency of the multiplex pcr
方法选定四个y染色体str基因座，应用加尾序列引物设计策略设计的引物，构建四个基因座的y - str复合扩增体系，建立银染检测和荧光检测方法，依据dna分析技术工作组( twgdam )指南进行法医学可行性研究和群体遗传研究。 |
| 8. | On the other hand , - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established . methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation . this vector is used to pronucleus microinjection
本实验以pegfp - n1质粒为骨架载体，用酶切连接的方法构建一个顺序含有- actin启动子、 fad2cdna 、 sv40polya加尾信号的真核表达载体，双切线性化后回收，使用回收的表达载体经原核显微注射生产转基因小鼠。 |
| 9. | The cdna is 2 149 bp long with an open reading frame of 1 697 bp , which encodes a polypeptide of 565 amino acids , preprotein of 62 . 3 kda ; 5 " untranslated regions contains 59nt , 3 " untranslated regions contains 391nt , a poly ( a ) tail signal and long poly ( a ) tail
该cdna全长2149bp ，包含一个1697bp的开放阅读框架，编码565个氨基酸。在起始密码子上游有一个由59个碱基组成的5 ’非编码区；在终止密码子下游有一个由391个碱基组成的3 ’非编码区，包括4个分解信号、 1个加尾信号和1个长度为17个腺苷酸的poly ( a )尾。 |
| 10. | Over 13 kb saci restriction fragment was cloned into pgem - 7zf ( + ) , mapped for restriction endonuclease sites and an about 5 . 0kb fragment was further subcloned and sequenced . through coding region specific primer , we amplied it ' s corresponding cdna , named st901 . st901 is 2889bp long , contains 1447bp putative promoter region within 5 " upstream and three exons ( 475bp , 140bp , 39bp ) and two introns ( 472bp , 2s3bp ) in the coding region , encodes a hydrophilic protein of 217 amino acid residues with a molecular mass of 24kda
以同源探针筛选马铃薯基因组文库，得到四倍体马铃薯基因组dna ? st901 ，基因全长2889bp ，含3个外显子(长度分别为475bp ， 140bp ， 39bp )和2个内含子(长度分别为472bp ， 253bp ) ； 5 ’端含有1447bp的启动子区段，该区段具备一般启动子的基本元件tatabox和caatbox ； 3 ’非编码区长63bp ，具hind酶切位点，没有发现保守的加尾信号。 |
