| 1. | Assay of the biological characteristics of transgenic sgc
转基因克隆株的生物学特性鉴定 |
| 2. | Study on gene expression profile of subclonal cell lines in two different anchorage - independent uterine cervical carcinoma
不同表型的人宫颈癌细胞亚克隆株的基因表达谱 |
| 3. | At first , study on antigenic variation in trypanosoma evansi from a clonal stock in rabbit was carried out
首先进行的是伊氏锥虫一个单克隆株在5只兔体内连续抗原变异的研究。 |
| 4. | ( 4 ) the mrna and protein expression grp94 were detected by rt - pcr and western bloting respectively to find the cell clones that have been transfected successfully . 2
( 4 )分别通过rt - pcr方法和westernblot方法从mrna和蛋白水平检测grp94的表达情况，以此鉴定转染成功的阳性克隆株。 |
| 5. | This result indicated that the virus pd70344v derived from the infectious molecular clone is a highly virulent strain which had inherited main characters from its parental virus dv
这一结果说明本文所构建的感染性分子克隆株pd70344v具有其亲本dv株的基本生物学性状，是一株具有高致病力的衍生病毒。 |
| 6. | Constructing human colorectal cancer cell line with stably down - regulated grp94 ( 1 ) the plasmid prc / rsv - ribol that contains specific grp94 - targeting ribozyme and the control plasmid prc / rsv were miniprepared , respectively , cleaved by endoenzyme pvuii
稳定下调grp94的人大肠癌细胞克隆株的构建( 1 )分别对含有特异性打靶grp94核酶的质粒prc rsv - ribo1和对照组质粒prc rsv进行小量提取、 pvu酶切鉴定。 |
| 7. | The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis
取阳性重组噬菌体抗体克隆株pcsa1 ， pcr扩增其scfv基因，筛选重组子进行序列测定，发现其序列符合小鼠抗体基因的一般特征，并且与几株抗磷酸胆碱的抗体重链和轻链可变区序列的同源性达80以上；推测pcsa1scfv针对的抗原是磷酸胆碱类物质。 |
| 8. | These results suggested that the 65kda protein was a degraded product , bioassay result showed that insecticidal activity of bl - 3 was 3 - fold higher than that of bl - 2 against 3rd instar plutelh xylostella . 3 . expression of cryla ( b ) gene in pseudom on a s fluoreseent pfx - 18 in order to study expression characteristics of icps gene in pseudom onas fluorescent , a new simple transformation method of pf was established
3 .用上述重组技术获得了含有刀口基因的lz20和含有尸2拼即“的lz20a克隆株，通过基因的诱导表达，发现p ”和pz浏民有不同的作用机制，证明了p20具有保护cyt人溶细胞的作用，这种作用并不依赖大肠杆菌受休的不同而改变 |
| 9. | A rabbit was infected with a cloned yntatl , blood was collecting from from the rabbit every 3 days after infection within 30 days , 10 clonal trypanosome populations were gotten , infecting a new rabbit by the last non - cloned trypanosome population . repeated above all , thus infected 5 rabbits sequentially . twenty different vats ( variant antigen type ) were monitored and characterized from those fifty mono - clonal populations by indirect immunofluorescence test ( ift ) and avidin biotin enzyme immunoassay ( abc - eia )
用伊氏锥虫云南水牛单克隆株yntat1感染兔，感染后30天内，每3天从兔血中分离锥虫并单虫克隆，最后一个未单虫克隆的虫株感染另一只兔，重复以上操作，这样顺序感染5只兔子，共获得50个单克隆锥虫种群( tp ) ，经间接免疫荧光和abc酶标试验鉴定共为20个抗原性互不相同的抗原变异体( vats ) 。 |
