English translation for "克隆扩增"
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- clonal expansion
Related Translations:
克隆动植物: cloning animals and plants
- Example Sentences:
| 1. | Thus , it is evident that 3t3 - l1 preadipocytes could differentiate into adipocytes without dna synthesis and mitotic clonal expansion 这些结果说明克隆扩增和dna复制并不是3t3 l1前脂肪细胞分化的必需步骤。 | | 2. | Further studies indicate that lipid rafts and caveolae are required only for igf - 1 receptor downstream signaling and not the activation of receptor 而非特异性的脂质结合试剂, xylazine ,不影响脂肪细胞分化和克隆扩增。 | | 3. | By inducing 3t3 - l1 preadipocyte with individual hormone , it was identified that the mitotic clonal expansion was induced only by insulin , not by 1 - isobutyl - 3 - methylxanthine or dexamethasone 有报道用药物抑制了克隆扩增的同时抑制了细胞的分化。我们发现克隆扩增是仅由胰岛素诱导引起的。 | | 4. | However , significant fraction of 3t3 - l1 preadipocytes differentiated into adipocytes without the mitotic clonal expansion when the preadipocytes were induced with the combination of 1 - isobutyl - 3 - methylxanthine and dexamethasone 用地塞米松和ibmx对细胞进行诱导不发生克隆扩增,而细胞也能部分分化,分化形成的脂肪细胞未经过dna复制。 | | 5. | Furthermore , when normally induced 3t3 - l1 preadipocytes were treated with pd98059 ( an inhibitor of mek - 1 ) , to block the activation of erk1 and 2 , the mitotic clonal expansion was blocked , while adipocyte differentiation was not affected 我们用有丝分裂原激活蛋白激酶激酶( mapkk / mek1 )的抑制剂pd98059与诱导分化激素及药物一起对细胞诱导分化,克隆扩增不发生,但细胞分化未受影响。 | | 6. | After the examination of pcr , one non - fluorescent cell clone had the same result as the plasmid ploxifn - a 1000bp product ; another non - fluorescent cell clone showed a 500bp product , a deletion reaction was thought to happen between the two lox sites in the genome under the reaction of cre recombinase so that the gfp gene and neo gene etc were cut 经pcr检测后,有一个不发光细胞克隆扩增出了与对照质粒ploxifn相同的约1000bp的条带,表明发生了替换反应;另一个不发光细胞克隆认为是在cre重组酶的作用下其内部自行发生了剪切作用,剪切了gfp基因,从而只扩增出了载体序列和一个lox位点的约500bp的条带。 | | 7. | Upon the differentiation induction of 3t3 - l1 preadipocytes by hormone cocktail containing 1 - isobutyl - 3 - methylxanthine , dexamethasone and insulin , the preadipocytes undergo ~ 2 rounds of mitotic clonal expansion , which just precedes the adipogenic gene expression program and has been thought to be an essential early step for differentiation initiation 诱导分化的早期细胞会进行约两轮的细胞分裂,被称为克隆扩增。克隆扩增以前一直被认为可能有利于染色体的重构以易化分化所需要的基因表达,因此克隆扩增可能是3t3 l1前脂肪细胞分化所必需的过程。 | | 8. | Disruption of lipid rafts and caveolae by depleting cellular cholesterol with cholesterol - binding reagent , - methylcyclodextrin or filipin , blocks the igf - 1 receptor signaling in 3t3 - l1 preadipocyte . both hormone induced adipocyte differentiation and mitotic clonal expansion are inhibited by lipid rafts and caveolae disruption . however , a nonspecific lipid binding reagent , xylazine , does not affect adipocyte differentiation or mitotic expansing 利用胆固醇结合试剂,如甲基环糊精和菲律宾菌素,减少细胞胆固醇的含量,破坏细胞膜上脂质筏/质膜微囊的结构,抑制了igf - 1受体信号诱导的3t3 - l1前脂肪细胞的分化以及克隆扩增。 |
- Similar Words:
- "克隆坎普" English translation, "克隆克" English translation, "克隆孔" English translation, "克隆库" English translation, "克隆扩充" English translation, "克隆来源" English translation, "克隆流产" English translation, "克隆流产假说" English translation, "克隆率" English translation, "克隆罗德" English translation
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