| 1. | This study provides scientific theoretical information for the molecular epidemiology of pseudorabies in guangxi and lays a good foundation for constructing ge gene delected vaccine and the diagnostic method of identifying prv
这些结果对我区伪狂犬病病毒的分子流行病学调查和构建ge基因缺失疫苗，建立伪狂犬病的鉴别诊断方法奠定了基础。 |
| 2. | Western - blot analysis showed the expressed protein was specific to antisera against prv fa strain . the ge - elisa for differentiation of prv infected from vaccination was developed using the expressed ge protein as antigen
Coli中高效表达的伪狂犬病病毒ge蛋白为抗原，以辣根过氧化物酶( hrp )标记的spa为二抗，建立了ge - elisa鉴别诊断方法。 |
| 3. | The transfer vector pbdtk - uni can be used for the construction of recombinant prv expressing foreign gene ( s ) . postgraduate : tianzhijun specialty : preventive veterinary science supervisors : prof . li yijing prof . tong guangzhi
以上结果表明所构建的具有自主知识产权的通用prv转移载体pbdtk - uni是成功的，为利用该载体构建伪狂犬病病毒二价基因工程疫苗提供了技术平台。 |
| 4. | Pseudorabies , c aused b y p seudorabies v irus ( prv ) , i s a d isease c haracterized b y neurological disorder in piglets and reproductive failure in pregnant pigs . the vaccines currently used in china and other countries are all with ge - phenotype
伪狂犬病病毒( pseudorabiesvirus ， prv )归属于疱疹病毒亚科，主要引起猪的伪狂犬病( pr ) ，该病以仔猪的神经学症状和妊娠母猪的生殖障碍为主要特征。 |
| 5. | Three pairs of primers were designed according to the sequences pulished by the genebank in order to amplifiy gd , ge and tk gene of the pseudorabies virus min - a strain . the gd , ge and tk gene were obtained by polymerase chain reaction ( pcr ) , and then cloned into the pgem - t easy vector
参考genebank收录的伪狂犬病病毒gd 、 ge 、 tk基因的序列设计了三对引物，对prvmin - a株进行了pcr扩增，扩增产物克隆于pgem - teasy载体。 |
| 6. | It revealed a negative reaction with positive sera of prv hcv , prrsv , jev and sa215 . it revealed a positive reaction with prv standand positive serum . the results showed that the established ge - elisa has the advantages shch as high sensibility strong specificity and good repeatability and can be used to differentiation of prv infection from vaccination
与猪细小病毒、猪瘟病毒、猪乙型脑炎病毒、 prrsv的标准阳性血清呈阴性反应，与伪狂犬病病毒标准阳性血清呈阳性反应，与三基因缺失疫苗株sa215免疫猪所采血清呈阴性反应。 |
| 7. | The gd and ge gene was subcloned into puc18 , resulting in pugdge . the fragment from pcdnas . 1 - including hcmv promoter / enhancer , mcs and neomycin resistance gene was inserted into the bamhi and bsteii restrication sites of pugdge , resulting in the universal transfer vector pgd - m - ge . the universal transfer vector pgd - m - ge has deleted the gi gene and 363bp in the 5 ' end of the ge orf of prv . there were 11 restrication sites for insertion of the foreign gene . the upstream and downstream flanking sequences were up to 1 . 25kb and 1 . 42kb . it will be useful for developing the recombinant prv expressing foreign gene ( s )
将gd 、 ge基因连接于质粒puc18获得pugdge ，缺失质粒pugdge的bamh和bste位点间391bp的片段。在此缺失位置插入来自质粒pcdna3 . 1 -的一伪狂犬病病毒gd 、 ge 、 tk基因的克隆与通用转移载体的构建段含hcmv启动子。多克隆位点和neo报告基因的片段，构建了通用转移载体ppd m pe 。 |
| 8. | Ie protein have multiple functions , such as conversion of the host cell into a metabolically activate states , activation of viral early genes in the early phase , repression of their own transcription . ie 180 proteins can stimulate transcription from both homologous such as the icp4 of hsv ( herpes simplex virus ) and heterologous viral and cellular promoter such as - globe protein
经分析伪狂犬病病毒立即早期蛋白( immediate - earlyprotein180 , ie180 )的蛋白质结构，发现ie180具有转录激活因子的结构特征，能与类rna聚合酶结合， ie180基因缺失突变体对sv40及cmv启动子调控作用的研究，是考察ie180能否作为转录激活因子应用的关键。 |
| 9. | Pseudorabies virus ( prv ) is the causative agent of aujeszky ' s disease , which results in significant losses in pig husbandry . thymidine kinase ( tk ) gene is one of the main virulent genes of prv , and it is essential to the propagation of prv in the nerve tissues , but dispensable for virus replication and infection in other tissues
伪狂犬病是由伪狂犬病病毒( pseudorabiesvirus , prv )引起的家畜和多种野生动物的一种以发热、奇痒、呼吸和神经系统疾病为特征的急性传染病，妊娠母猪可发生死胎和流产，是危害养猪业的一个重要传染病。 |
