清夜扪心: examine one's conscience in the stillness of night -- self-examination; search oneself for mistakes in the stillness of night
Example Sentences:
1.
Fusion expression of m - centrin in e . coil bl21 was performed by induction of fptg . fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography . the final products were checked by sds - page gel 融合蛋白gst - m - centrin菌体经过超声波裂解后得到的上清夜经过gst亲和层析后用prescissionprotease ( ppase )酶切，酶切产物再次经过gst亲和层析和hitrapq阴离子交换层析两步柱层析纯化后，得到纯度较高的的m - centrin 。
