| 1. | But the routine methods of yac transgene transfer such as microinjection cannot introduce yac into livestocks by now
但目前还没有报道用常规的yac转基因方法能成功的将yac导入大动物体内的例子。 |
| 2. | To clone large and random dna fragments , the second generation of yac , pjs97 - pjs98 was modified as gene replacement vector in streptomyces
4kb和1 . okb片段，而且还插入了勿，七刃j和spc心t厂抗性基因。 |
| 3. | For desired gene replacement , hyg / cml and str / spc gene cassettes were also incorporated into the new vector system . it is expected that hygwould be integ
为了验证新型yac载体系统的功能，我们将phz621 ~ phz622进行了适当地改造，构建了质粒phz625 。 |
| 4. | In order to construct plant artificial chromosome , plasmid vectors have been constructed to integrate necessary elements into both right and left yac arms
为了构建植物人工染色体，我们构建了可以和含有着丝粒序列的yac载体的左臂和右臂进行同源重组的质粒载体。 |
| 5. | Phz621 - phz622 , the new gene replacement vector system , had been constructed through the insertion of the 1 . 0kb and 1 . 4kb flanking sequences of hau3r gene from wild - type s . lividans in the same natural orientation
预期在携带有大插入片段的基因置换yac分子进入野生型变铅青链霉菌后， yac分子将通过1 . 4kb或1 |
| 6. | The chief aim of the present study is trying to extend the applications of yac and scp2 * , which have tremendous cloning capacity , for the above mentioned purpose . streptomyces sp . fr - 008 pks gene cluster ( c
本文对yac和scp2 *这类承载能力较大的载体系统在克隆fr - 008抗生素合成基因簇方面的利用进行了一系列载体改造和克隆策略方面的初步探索。 |
| 7. | It have been reported that the 210 kb yeast artificial chromosome ( yac ) including the human a - lactalbumin gene could direct the transgene to express in a position - independent manner , therefore is an excellent expression vector to produce transgenic animal bioreactors
根据报道含完整人-乳白蛋白基因的yac载体能指导外源基因的乳腺特异性高效表达，是生产动物乳腺生物反应器的理想表达载体。 |
| 8. | Isolation and purification of modified yacs from yeast strain have been carried out by pulsed - field gel electrophoresis . the modified yacs are expected to generate plant artificial chromosome after being transferred into arabidopsis protoplast by liposome - mediated method
用脉冲电泳将经过同源重组的修饰的yac克隆分离出来通过脂质体转化植物的原生质体，希望得到在植物细胞中稳定存在的植物人工染色体。 |
| 9. | Because of its fully sequenced genome and the most complete , contiguous nucleotide sequence of centromeric dna in plants , arabidopsis was chose as the base to construct plant artificial chromosome . we identified two candidate yac clones , cic8h8 and cic6f6 , which are both proved to have the 178 bp arabidopsis centromeric repeat sequences
我们通过pcr和序列分析，选择了含有拟南芥着丝粒区178bp串连重复序列的yac克隆作为构建人工染色体的基础，同时也克隆了拟南芥的端粒和ars等序列。 |
| 10. | To facilitate the detection of gene replacement , apr gene was placed in the middle of the two inserts . orit from e . coli plasmid rk2 was also incoporated into the vector , therefore , pij903 derivatives can be mobilized from e . coli into streptomyces sp . fr - 008 by either bi - parental conjugation or by conjugation between streptomyces
为了在克隆完整基因簇时避免这两方面的影响并能随机地获得dna大片段，我们将第二代yac载体pjs97 - pjs98改造成为适合于在白林泉链霉菌基因组中dna大片段的基因置换与克隆链霉菌中分析被克隆大片段功能的基因置换载体。 |
