Temporal patterns of vitellogenin ( vg ) synthesis and uptake as well as vitellin ( vn ) degradation in n . vitripennis , were studied using the method of enzyme - linked immunosorbent assay ( elisa ) 采用elisa测定法检测了丽蝇蛹集金小蜂卵黄原蛋白合成与摄取，及卵黄磷蛋白降解的时间动态。
2.
In this paper , we selected a widely used organophosphorous pesticide - - monocrotophos , used goldfish as model animal , and chose the vitellogenin induction of male fish as the main indicator , - gtp of sertoli cell in testes , the changes of microstructures of testis and ultrastructures of pituitary and testis as assistant indicators , we finally built up a screening system to effectively detect the environmental estrogen effect of organophosphorous pesticides , and reported that the monocrotophos had the activity of environmental estrogen for the first time in the world 因此，本文选择国内广泛使用的有机磷农药? ?久效磷，以金鱼为模型生物，以雄性金鱼卵黄原蛋白产生、精巢支持细胞- gtp酶活性，并辅以生殖轴线器官? ?垂体和精巢超微结构的变化，在国内首先建立了有机磷农药环境雌激素效应的筛选体系，并首次确认久效磷具有环境雌激素活性。
3.
In this paper , the ovarian development , oogenesis , vitellogenin molecular characteristic , vitellogenin synthesis and uptake , and vitellin degradation in nasonia vitripennis ( hymenoptera : pteromalidae ) , are studied . the detailed results are shown as follows : 83 % of ovaries were found to be composed of eight ovarioles with four on each side and 17 % of ones to be composed of six ovarioles with three on each side . ovarioles appeared on the 10th day after parasitization , which were transpearant and had no egg chambers 为此，本论文就蝇类蛹期外寄生蜂? ?丽蝇蛹集金小蜂nasoniavitripennis (膜翅目：金小蜂科) ，卵巢发育、卵子发生，卵黄蛋白的分子特性，及卵黄原蛋白的合成、卵巢对卵黄原蛋白的摄取及卵子内卵黄磷蛋白的降解动态作了研究，结果如下：丽蝇蛹集金小蜂卵巢83由4对卵巢管组成， 17由3对卵巢管组成。
4.
The results of native - page showed that the male fish could synthesis vitellogenin after the injection of 0 . 05mg g " ' bw of 17 - estradiol for two weeks . the average content of vitellogenin in ej exposed group was 690 . 2ng ml - 1 by elisa , it was significantly different from male control group ( p < 0 . 01 ) , which average content was 10 . 7 ng ml - 1 , and it was also much higher than the female control group , which average content was 285 . 5 ng ml - 1 . compared with control male goldfish , both the ca2 + content and total protein content in serum of the e2 exposed male goldfish showed significant increase 电泳结果表明，经过2周的17 ?雌二醇暴露，诱导雄性金鱼产生了卵黄原蛋白，并通过elisa检测卵黄原蛋白的平均含量为690 . 2ngml ~ ( - 1 ) ，和对照组雄性金鱼平均含量为10 . 7ngml ~ ( - 1 )的差异极显著( p 0 . 01 ) ，比雌性对照组检出量285 . 5ngml ~ ( - 1 )高1倍多，并且17 ?雌二醇可以诱导雄性金鱼血浆钙离子和血浆总蛋白含量增加。
5.
For each fedding treatment , the longitude and latitude of the root egg , the number of egg chambers with each developmental grade all changed in a parabola model . the results from native page indicated that there were two specific protein i . e . vitellogenin ( vg ) or vitellin ( vn ) in the female wasp of n . vitripennis 同一种喂食处理中，卵巢长度的时间动态几乎都无变化； 1级卵子数、 2级卵子数和成熟卵子数的变化，是抛物线变化趋势，即先增加，然后减少；基部卵子短径和长径也是抛物线变化趋势。
