| 1. | Bacterial transposons carry genes encoding antibiotic resistance .
细菌转座子可以携带对抗生素的抗性基因。 |
| 2. | Sequences flanking tn5 in the mutants were cloned by self - ligation . because each transposon contains an origin of replication functioned in e . coli , but not in rhizobium
使用质粒自连法克隆了042bm - x1和042bm - x2中tn5插入位点的侧翼序列。 |
| 3. | The dissertation mainly concerns the construction of resolution vector based on bacillus thuringiensis transposon tn4430 . the research results are summarized as following : 1
本论文主要围绕构建苏云金芽胞杆菌解离载体而开展研究工作，研究结果如下： 1 |
| 4. | Insertional mutagenesis using t - dna , transposons and plasmids axe commonly methods to creating a mutant library . here we summarize these strategies as well as the progress in the functional genomes research
本文分别介绍三种方法的原理及其在功能基因组学研究中的应用和研究进展。 |
| 5. | Two magnetosome deletion mutants were constructed by conjugative transposon mutagensis and the application of this genetic system . the two magnetosome deletion mutants were named as nm4 and nm21 respectively
并利用此体系，通过接合转座诱变技术，获得了2个磁小体缺失突变株： nm4 、 nm21 。 |
| 6. | A mutant library of xanthomonas campestris pv . campestris ( hereafter xcc ) strain 8004 with 17820 clones was constructed by random transposon tn5gwsa5 mutagenesis , which cover 1800 predicted orfs of xcc genome
用转座子tn5gusa5诱变野油菜黄单胞菌( xanthomonascampestrispv . campestris以下简称xcc ) ，构建了xcc8004tn5gusa5插入突变体库。 |
| 7. | Rna silencing is a common phenomenon of rna degradation that is induced by homologous sequences . virus and transposon invasions and various kinds of aberrant rnas can provoke rna silencing
Rna沉默是生物体中普遍存在的一种由同源序列引起的rna降解过程，病毒或转座子入侵、以及体内产生的各种异常结构rna都可能成为诱导rna沉默发生的因素。 |
| 8. | A new resolution vector based on tnpi - mediated site - specific recombination system of b . thuringiensis transposon tn4430 was developed . the crylac10 or other target dna , and the gene ori1030 , from a plasmid of wide type b . thitringiensis subsp
利用转座因子构建解离载体的可行性利用苏云金芽胞杆菌转座子tn4430的解离区构建了解离载体。 |
| 9. | This paper summarizes the commonly used methods for the research of gene function of filamentous fungi , such as transposon tagging , gene knockout , rna interference , over - expression and yeast hybrid system , and provides a discussion on the advantages and disadvantages of those methods
本文总结了目前丝状真菌基因功能研究中常用的方法，如转座子标签法、基因敲除技术、 rna干扰、超表达及酵母杂交系统等，并对各方法在丝状真菌研究中的优缺点进行了阐述。 |
| 10. | By comparing the biological characteristics of original normal filament , linear filament and the curved filament retransited from linear filament , certain evidence of the morphologic variation regulated by a special transposon are detected on the level of protein and dna , which will help us to discover the mechanisms of this morphologic variation on molecular genetics level and solve the problem in production of spirulina in large scale
在比较了正常藻丝体、变直藻丝体及回复正常螺旋形态的藻丝体一组材料生物学特性的基础上，进一步在蛋白质及dna水平上找到了转座子调控此形态变异的某些证据，为阐明螺旋藻形态变异与重建的分子遗传学机制以及解决螺旋藻大规模生产的实际问题提供理论依据。 |
