| 1. | And a atf3 - bd reduced the activity of interaction with restin . it suggested that restin most likely interacted with atf3 binding domai
激活的atf3可以通过jnk / sapk信号传递途径抑制细胞内部分基因的转录和细胞的增殖。 |
| 2. | The phylogenetic analysis of mage family proteins and restin shows that the relationship of restin is nearer to type ii , such as mage - d1 and necdin , rather than type i mage members
进一步的分析发现， restin定位于xp11 . 22 ，开放读框全部位于一个外显子中，这同绝大多数mage基因的定位特点及基因组结构一致。 |
| 3. | The tissue expression analysis of restin shows that restin same as necdin and mage - d , is high expressed in prostate , testis , nervous tissue and fetal tissues , and low or less in the white blood cells and tumor tissues
而在肿瘤组织中不表达或表达量极低，这与h类mage家族成员mage一d 、 necdin表达分布规律相一致。 |
| 4. | Chomez and colleague also found this gene by homologous sequence splicing in the database of gene bank in 2001 and named as mage - hi , but there are no any biological functions of restin reported
2001年， chomez等通过同源序列筛选genbank数据库，也发现了该基因，并命名为mage - h1 ，但有关该基因的具体生物学功能研究还没有报道。 |
| 5. | A lot of transcription factors such as atf , c - fos , c - jun , e2f and p53 were envolved in this stage . in this study , we try to identify the transcription factors interacted with restin by using mammalian cell two - hybrid system
当细胞自静止状态的go期进入gi期时，众多转录因子如灯f 、 e一fos 、 e一jun 、 ezf 、 p53等均参与这一进程。 |
| 6. | Morever , tcherpakov and colleague found that the mage - d1 , necdin and mage - hi ( restin ) could interacted with p75 . so , we deduced that the restin most likely regulated cell cycle by interacting with some transcription factors
Restin与necdin同源性最高，组织表达一致且都具有细胞生长抑制作用， teherpakov等也发现mage家族分子mage一nl 、 needin和mage一hx ( restin )都可以和p75相作用。 |
| 7. | The results showed that all six tfs displayed no autoluminescence in this two - hybrid system , and one of them , the atf3 displayed a strong interaction with restin . to verify the interaction of restin and atf3 , a series of truncated fragments of atf3 were inserted into pact to be used in the further study
三、为了进一步验证atf3与restin的相互作用，对atf3进行截断并构建了一系列atf3的截短片段的双杂交质粒(仍然构建于pact载体中) ，再次运用细胞双杂交进行相互作用的探索。 |
| 8. | The tumor cells transfected with restin are arrested in gl phase and the cell proliferation is inhibited . it implies that restin may be correlated to cell cycle regulation . other data showed that the necdin , a homolog of restin , could suppress cell growth by interacting with transcription factor e2f1 and p53
而与restin的同源蛋白的necdin ，可以与细胞周期促进蛋白sv40大t抗原、腺病毒eia 、转录因子ezfi等相互作用，抑制细胞生长;也可以和转录因子p53作用参与生长抑制和抑制p53诱导的细胞凋亡。 |
| 9. | Restin was a novel gene isolated by our lab from differentiation tumor cell induced by all - trans retinoic acid in 1999 , and its molecular functions were not well understood . it mainly expresses in terminally differentiated cells . by transfection of restin into tumor cell , the cells were arrested in g1 phase and the cell proliferation was inhibited
Restin是1999年本室在研究全反式维甲酸诱导细胞分化时克隆得到的一个人类未知功能的新基因，该基因主要表达于终末分化细胞，稳定转染该基因的细胞出现g1期阻滞，抑制细胞的增殖，提示restin与细胞周期的调控有关。 |
