Renature the purified target fusion protein through multistep dialysis . then unwater the frozen protein in order to store 本序列作为自中国人克隆的hpd一3被genbank登录，登录号为af516673 。
2.
After that a good renature result was obteined using gradient with lower protein concentration . the purity of the recombinant protein by affinity - purification of glutathione sepharose 4b and electrodialysis method was also attained perfect result . the antigenicity of the gst - ha1 protein were identified using elisa mathod 用梯度透析法对变性的融合蛋白gst - ha1进行复性，在低蛋白浓度的条件下，获得了很好的复性结果，复性前蛋白浓度为1 . 02mg ml ，复性后蛋白浓度为0 . 875mg ml 。
