Sayyid qutb also said that balance dominates the universe 古图布也说： “宇宙里统领一切的是均衡。 ”
2.
2 . cloning and functional expression of a heterogenous gene from a . nidulans in e . coli strain a . nidulans chromosome dna was used as template to amplfy gene qutb by pcr , the dna sequencing showed that the cloned gene was in agreement with the reported sequence 异源奎尼酸脱氢酶( qdhase )在大肠杆菌中功能性表达以构巢曲霉菌( a . nidulans )基因组dna为模板，用pcr方法扩增得到了qutb基因，序列测定与文献报道一致。
3.
Four of the six resulting recombinants contained two genes arog and qutb , the other contained three genes arog , qutb and arob . the catalytic activities of both ds and qdhase increased diffierently , but enzyme activities of the recombinant harboring pbv - prpl - qutb - pl - arog were best 六种重组子都实现了目的蛋白的表达，表达产物ds和qdhase酶活性有不同程度的提高，其中pbv - prpl - qutb - pl - arog串联表达重组子表达产物二种酶活性ds和odhase都比较高，是优化的重组子。
4.
Quinic acid , used shikimate pathway in e . coli , it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell . double specific enzyme genes arog , qutb or three ones arog , qutb , arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions . modifications of e . coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch 利用大肠杆菌莽草酸途径合成新的代谢物奎尼酸，须在宿主细胞引入异源酶基因扩展代谢途径；串联表达酶基因，同时适量增加不同种属的多个关键酶酶量，改善限速反应；利用同源重组进行基因整合和基因破坏，改造染色体结构定向改变微生物代谢途径；目的是将碳代谢流最大程度的引向奎尼酸生成的方向。
5.
The gene qutb was cloned to pbv220 , qdhase was expressed and had specific activity in e . coli . 3 . construction of co - expression recombinants of multigenes in a single plasmid pbv220 and its optimization according to different combined modes , six co - expression recombinants , each gene of which recombinant is under control of lambda phase prpl or pl promoter , were constructed and expressed 单质粒pbv220多基因串联表达重组子的构建及优化以不同方式组合，构建了含有各自启动子prpl或pl的二基因arog 、 qutb串联表达重组子四种和三基因arog 、 qutb 、 arob串联表达重组子二种。
6.
When sultan qutb - ud - din aibak 1150 - 1210 ce shifted capital from lahore to delhi in 1193 , hundreds of thousands of people - soldiers , scholars , writers , tribes , merchants , government employees , artists , sufis and others - migrated en masse with him and took this proto language with them 当sultan qutb ud声浪aibak 1150 - 1210 1193年转移了资本从lahore到德里,成千上万的人民-战士学者作家部落客商政府职工艺术家, sufis和其他人-集体移居与他和采取了这种proto语言与他们。
