K01458 , 1284bp ) , we designed the primers and regard the pcr productions as the internal standard . then by using rt - pcr analysis , high levels of expression of emu ghrelin mrna were detectable in proventriculus were detected , low levels of expression of duck ghrelin mrna were also detectable in lung muscle ileum duodenum corpus striatum cerebellum brain stem and gizzard K01458 ， 1284bp )设计引物，以其pcr产物作为内标，分别以鸸鹋各个组织反转录产物为模板，通过多重pcr的检测ghrelin的mrna在鸸鹋的各个组织中表达情况，结果发现ghrelin的mrna在鸸鹋ghrelin的mrna除在腺胃特异表达外，在肺、肌肉、回肠、十二指肠、纹状体、小脑、脑干、肌胃也有少量表达。
2.
In this paper , a field strain of infectious bronchitis virus was isolated from proventriculus tissue , morphological observation by electron - microscope and the biological characterizations of the virus were studied , pairs of specific primers are designed and synthesized in correspondence with them , according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes , the cdna of si gene , s2 gene , m gene . n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported 在此基础上，根据国内外已发表的ibv基因序列，分别设计特异性引物，应用不同引物进行反转录合成cdna ，分片段对ibv的主要结构基因进行pcr扩增，并分别将各个目的片段克隆到puc19载体上，在大肠杆菌dh5中实现目的基因的分子克隆，经蓝白斑筛选、限制性内切酶分析、 pcr鉴定，筛选出重组阳性质粒，并对各个目的基因片段进行序列测定，从而获得ibv主要结构基因全序列。
3.
Ghrelin regulates pituitary growth hormone ( gh ) secretion . in the study , we obtained the cdna sequences of preproghrelin and the cdna encoding the ghrelin of duck , goose and emu by using rt - pcr and 3 " - race methods . and then deduced the amino acid sequences of preproghrelin and ghrelin in duck . goose and emu . sense and antisense primers were designed on the basis of chicken ghrelin cdna sequence ( dest accession no . ab075215 , 836bp ) and proventriculus cdna was performed as the template Ab075215 ， 836bp )设计引物，以鸭、鹅、鸸鹋腺胃cdna为模板，通过rt - pcr及3 - race的方法，将各个产物经过回收，再经连接转化，克隆测序，拼接后得到鸭、鹅、鸸鹋preproghrelin基因的cdna序列及ghrelin的cdna序列，并由此推测出preproghrelin及ghrelin的氨基酸序列。
