A 1 . 7kb fragment encoding ge of prv fa strain was obtained by pcr from plasmid ppge templated using a pair of the designed primers containing ecori and bamhi ' sites . the ge gene fragment cutted with ecori and bamhi was inserted into the expression plasmid pbv220 including these two endonuclease sites for constructing the recombinant plasmid pbvge . strain dh5a of e . coli contain pbvge was induced at 42 for 4 - 6hr after incubation with vigorous shaking at 30 for 3hr or so 以质粒ppgedna为模板， pcr扩增出1 . 7kb的ge基因完整片段，将扩增产物以ecori和bamhi双酶切后，插入原核表达载体pbv220的p _ rp _ l启动子下游的ecori和bamhi位点间，得到重组表达质粒pbvge ，转化了pbvge的大肠杆菌dh5a经温敏诱导表达后，用sds - page和western - blot ，以及琼脂双扩散来检测，结果表明prvfa株ge基因在原核载体上得到高效表达，表达产物约占总蛋白的17 。
