The effect of ca2 on the proliferation of saccharomyces cerevisiae and schizosaccharomyces pombe 对酿酒酵母与粟酒裂殖酵母细胞增殖的影响
2.
Gene engineering based on reducing na + toxicity creats a new approach to plant salt tolerance . this is the first time expressing of sod2 in arabidopsis thaliana and maybe attribute to research the ion homeostasis in the cell . the sod2 gene was isolated from s . pombe by rt - pcr method and confirmed by sequencing 虽然sod2基因已经克隆，但是sod2在高等植物中异源表达及对其影响还未见报道，因此sod2基因在拟南芥中过量表达，对研究细胞的离子均衡及运用基因工程手段有效地提高植物的抗逆性具有重要意义。
3.
In order to study the mechanism of the effect of low concentration tfp on the proliferation of s . pombe , we watch yeast cells loaded with fluo - 3 under laser scanning confocal microscope ( lscm ) . the fluorescence intensity reflected the cytosolic free calcium concentration . the result showed that , the cytosolic free ca2 + concentration in s . pombe cultured in ca2 + - free medium was 2 ~ 3 times lower than that in s . pombe cultured in medium containing 10umol / l ca24 , while ca2 + concentration in s . pombe treated with 50umol / l tfp was 4 - 5 times higher 本文发现增加胞外钙浓度以及低浓度( 20 100 mol l )三氟拉嗪( tfp )不但能促进野生型s . pombe细胞的增殖，而且对mfp7菌株也有同样的效应，这说明胞外ca ~ ( 2 + )和低浓度tfp对不同遗传型裂殖酵母细胞的增殖均有促进作用。
4.
Sod2 from schizosaccharomyces pombe , which encodes a protein with homology to bacterial and mammalia na + / h + antiporters in plasma membrane and responses to efflux of na + , is an important gene i nvolving in homeostasis . though sod2 gene has been cloned , ectopic expression of sod2 in high plants has not been reported Sod2在裂殖酵母( schizosaccharomycespombe )中编码位于质膜上的na ~ + h ~ +逆向转运蛋白，控制na ~ +外排，对于保持裂殖酵母细胞的离子均衡非常重要。
5.
We used fission yeast schizosaccharomyces pombe ( s . pombe ) , an unicellular eukaryotic organism , as research material . electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ) , we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell . flow cytometry provided a way of determining the relative dna content of populations of fission yeast 本文以单细胞的真核模式生物裂殖酵母( schizosaccharomycespombe )为研究材料，通过激光扫描共聚焦显微镜观察酵母细胞胞质内游离ca ~ ( 2 + )的分布及相对浓度，以及不同周期时相细胞中gfp - cam的荧光强度变化，并采用细胞流式法对酵母细胞的相对dna含量进行测定以确定细胞所处周期时相。
