The detection of ribozyme gene with two cleavage sites that cleaves plrv replicase gene were present in the second part of this paper . a conserved sequence of 35s promoter of plrv was found in gene pools . two primers were designed based on the conserved sequence and bamh i and ribozyme gene . the genomic dna of potato was amplified by the primers through polymerase chain reaction ( pcr ) 从许多资料中报道的plrv的35s启动子的序列中找出一段保守序列，然后根据与核酶紧密相连的bamh和这段保守序列设计两段引物，用这两段引物通过pcr扩增转基因马铃薯的基因组dna ，并进行检测。
