The study on the function and mechanism of phrip1 is important for clarifying how the cell plate and cell wall form in plants . in this study , full length of phrip1 is amplified by pcr and ligated into pks plasmid , then the bait plasmid , peg202 - phrip1 , is constructed . the inseret gene are sure to be translated into the right fusion protein through its sequence . in the yeast two - hybrid system , the bait plasmid ( peg202 - phrip1 ) and a reporter plasmid ( psh18 - 34 ) are introduced into the yeast ( egy48 ) by co - transformation . then cdna library ( which is in pjg4 - 5 ) is screened and two genes are obtained . the two insert gene fragments are sequenced . one of them is plastocyanin , the other is putative photosystem i reaction center subunit ii precursor , both of them are the necessary components of photosynthetic chain 成膜素相关蛋白1 ( phrip1 )是一个含608个氨基酸的蛋白质，它对于植物胞质分裂中细胞板的形成起到了十分重要的作用。研究phrip1的功能和机制，对在分子水平上阐明植物细胞板以及细胞壁形成的机理具有重大的生物学意义。在本实验中，根据phrip1的序列设计引物对其进行pcr扩增，得到该基因后将其连接到了pks质粒上，并进一步构建成了诱饵质粒peg202 - phrip1 。
