Performance of the chinese hamster ovary cell hypoxanthine guanine phosphoribosyl transferase gene mutation assay 中国仓鼠卵巢细胞次黄质鸟嘌呤转磷酸核糖基酶基因变异鉴定的操作
2.
Histidine a basic amino acid formed only rarely in proteins . it is formed from atp and 5 - phosphoribosyl pyrophosphate in a complex biosynthetic pathway . glutamate is an intermediate in its breakdown 组氨酸:是蛋白质中少有的一种碱性氨基酸。由atp和5 -磷酸核糖基焦磷酸盐通过复杂的生物合成途径合成。在其降解过程中可形成谷氨酸。
3.
2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6 . their antibodies " isotypes are tested by goad anti - mouse isotype regent 取驯化好并处于对数生长期的2 - e _ 4 - a和s _ 2 - b细胞,再常规融合和筛选,三次克隆化后得稳定分泌双特异性抗体的杂交-杂交瘤细胞株6株。