| 1. | The screnning and prenatal gene dignosis for fragile x syndrome using pfu dna 综合征的筛查和产前基因诊断 |
| 2. | Plaque forming units , pfu 噬斑形成单位 |
| 3. | Ecological basis and application of pfu protozoan community in bio - monitoring with relation to water quality 原生动物群落生物监测的生态学原理与应用 |
| 4. | Phytoplankton was dominated by diatom and blue - green algae according to density and by diatom in light of biomass 利用pfu采集周丛原生动物60种,一半以上是耐有机污染的指示种。 |
| 5. | Vaccination trials were carried out in spf chickens , protection index ( pi ) against md5 + rbib challenge induced by 106 pfu rfpv - gb - ha was 53 . 8 while pi against aiv challenge induced by 106 pfu rfpv - gb - ha was 69 . 5 综上所述,本论文尝试了不同禽病的主要保护性抗原基因在鸡痘病毒载体的联合表达,为探索基因工程联苗打下了基础。 |
| 6. | The burst size of the cyanophage was ca . 200 pfu ? cell " 1 . the rate of adsorption to host was markedly higher under light condition than that under dark condition ( p = 0 . 02 ) , and light was necessary for infection 此外,固体培养条件下,织线藻的生长速率与噬藻斑的扩大之间有明显的同步性,即生长速度越大,噬藻斑的扩大就越明显。 |
| 7. | Several methods for measuring the lysing cycle and burst size of the cyanophage were tested . it was established that cell counting method was more convenient and exact for confirming the lysing cycle . burst size obtained by 1 pfu infecting was reliable 我们研究了确定该噬藻体裂解周期和释放量的多种方法,认为细胞计数法是测量裂解周期既方便又准确的方法,而采用1pfu的感染可获得噬藻体的准确释放量。 |
| 8. | In our experiment , the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ) , then sequenced . the cloned sequence was completely identical to the sequence which was issued in genbank 本实验采用了高保真pfudna聚合酶,在退火温度61条件下从转基因bobwhite品种基因组dna中扩增出特异性片段,将此片段插入克隆载体pgem - 7fz ( + ) ,经测序和序列分析表明,所扩增得到的片段含有bar基因完整的读码框,并且序列与genbank中发表的序列完全一致。 |
| 9. | Results we construct recombinant angiostatin baculovirus with a high virus titer ( 2 + 108 pfu / ml ) successfully . recombinant angiostatin was effectively expressed in insect cells ( sf9 ) as 53 kd fusing protein and its expression level was about 90 % of insect cellular total soluble proteins . the recombinant angiostatin protein could inhibit endothelial cell proliferation in vitro with ic50 value of 2 实验结果我们成功地构建了滴度高达2x10 ‘ pm llil的angiostatin重组? 2 ?杆状病毒,并在昆虫细胞sffi中高效表达了分子量为53kd的an giostatin重组蛋白,重组angiostatin蛋白不仅在体外显著抑制内皮细胞的生长, k 。 |