English translation for "pf4"

血小板因子Ⅳ

Example Sentences:

	1.	3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa . 2 after sds - page and densitometric scan analysis , the result show that expression level is 25 - 30 % of total bacterial proteins 山西医科大学2002届硕士学位论文一2dhsa pbv220 rhpf4经温控诱导表达后， sds page及凝胶密度扫描分析，表达产物占总国体蛋白的25 30 ，凝胶迁移特性与hpf4标准品相同。
	2.	Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully . 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr . after sds - page and densitometric scan analysis , the expression level of r hpf4 is 25 - 30 % 结论本研究运用pcr定点突变技术，完全去除了hpf4cdna基因3 ”端utrat富含区：改用大肠杆菌强串联终止密码子taaaataa ，成功构建高效表达克隆pbv220 rhpp4 。
	3.	Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells , we design a pair of particular primers , and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic . the expression plasmid was identified with pcr and dna sequencing . pbv220 - r hpf4 was transformed into e . coli dh5a , bl21 ( de3 ) and induced by increasing the temperature to 42 . we identified the expression protein by sds - page and western - blotting 目的1人血小板因子4 ( hpf4 )原核高效表达克隆的构建2重组hpf4的表达及分离、纯化工艺研究3重组hpf4的特性研究方法根据原核细胞表达真核蛋白的基因表达调控特点，设计合成一对特异引物，在pt7 - 7 - rpf4表达质粒的基础上，应用聚合酶链式反应( pcr )对其cdna进行改造，通过dna重组技术构建成重组hpf4原核表达质粒pbv220 - rhpf4 ，用快速pcr检测法、 dna测序分析，鉴定重组hpf4表达质粒的正确性。
	4.	Western - blotting result demostrated rhpf4 had specific reaction with rabbit anti - hpf4 antibody . our system improve the expression level of r hpf4 by 80 fold compared with pt7 - 7 - r hpf4 . after purified and renatured , r hpf4 prepared by our methods has bioactivity like wide hpf4 . our study establish a stable base for further reseach of the h pf4 and provide a theoretics gist for modulative mechanism of eukaryotic protein expression in prokaryotic cells 我们构建的rhpn原核高效表达系统经m page及凝胶密度扫描分析结果表明， rhpf4表达量占菌体总蛋白量的25 30 ，较原表达克隆pt7 7 rhpf4提高了近80倍，经快速高效的包涵体分高纯化工艺和复性工艺， rhpf4具有野生蛋白活性。

Similar Words：

"pf2丝形病毒" English translation, "pf2丝形簿" English translation, "pf3释放试验" English translation, "pf3丝形病毒" English translation, "pf3丝形簿" English translation, "pf4 ria" English translation, "pf=personal fouls" English translation, "pf=planned finish date" English translation, "pfa" English translation, "pfa perfluoro" English translation