| 1. | The insertion sites of tn5gusa5 were located by methods of tail - pcr and sequencing . for these 53 protease mutants , the insertion sites were distributed in 14 orfs
运用tail - por和测序的方法定位了转座子tn5gusa5的插入位置，经分析共插入14个orf中。 |
| 2. | All of three orfs were demonstrated to be necessary for the dnd phenotype . for the survey of the biological relevence , different 5 "
为了验证这三个基因与dnd表型的关系，构建了一系列衍生于整合型载体pset152的质粒： phz2150 ， phz2151 ， phz2152和phz2153 。 |
| 3. | Using erase - a - base system , a series of exoiii progressive deletion mutants were prepared for sequencing dnd cluster . analysis of the sequence revealed that it contained three orfs
为了对dnd功能区域进行序列测定和分析，通过exo缺失实验，获得了38个单向渐减缺失亚克隆。 |
| 4. | A mutant library of xanthomonas campestris pv . campestris ( hereafter xcc ) strain 8004 with 17820 clones was constructed by random transposon tn5gwsa5 mutagenesis , which cover 1800 predicted orfs of xcc genome
用转座子tn5gusa5诱变野油菜黄单胞菌( xanthomonascampestrispv . campestris以下简称xcc ) ，构建了xcc8004tn5gusa5插入突变体库。 |
| 5. | The derivatives of phz2104 resulting from the disruption of the three putative orfs respectively by aada can not confer dnd phenotype on zx1 . using erase - a - base system , a series of exoiii progressive deletion mutants were prepared for sequencing of add gene cluster
将add基因簇亚克隆到测序载体pbluescript sk ( + )上，采用exo缺失突变法得到了66个单向递减缺失亚克隆，然后对这些克隆进行测序。 |
| 6. | Lividans strains with dnd + and dnd " phenotype were used for testing phage infections , growth under different ph , temperature , concentration of nacl and an extremely low concentration of o2 . no obvious differences could be tested from these strains
它们均携带包含3个orfs的区域(除了phz2153在dndb和dndc内缺失了2kb ) ，但已分别在各orf上发生单基因中断( phz2150 ， phz2151和phz2152上外源片段的中断分别发生在dnda ，月和c上) 。 |
| 7. | Phaa , phab and phac were amplifed from the subclone of pseudomanas sp . producing pha by pcr . the gel electrophoresis analysis showed that the molecular weights of cloned phaa , phab and phac were equal to fragment speculated from three orfs
利用所提取的开放阅读框架的序列设计三对引物，采用pcr技术，从合成pha的亚克隆片段中分离出phaa 、 phab和phac三个基因片段，经凝胶电泳分析表明，所克隆的三个基因分子量大小与推测的三个开放阅读框架中基因片段大小一致。 |
| 8. | This unusual modification causes wild type s . lividans 1326 dna sensitive to site - specific oxidative double - strand cleavage ( dnd phenotype ) . preliminary study revealed that such dna modification involves incorporation of sulphur . the entire functional dnd cluster , 8 . 3kb in size , including 3 orfs - dnda , dndb and dndc was involved in this dna modification
野生型变铅青链霉菌具有一种不同于甲基化修饰的新型dna硫修饰系统，使dna在电泳时易遭到氧化双链切割而导致降解( dnd , dnadegradation )表型( zhouetal . , 1988 , 1994 , 1999 ) 。 |
| 9. | A small cryptical plasmid pefr was isolated from enterococcus faecium strain df101 . the complete sequence analysis of the plasmid show that it consists of 3176 bps , which contains four putative orfs . orf1 encodes a putative protein and is highly similar to repa which functions in replication
从屎肠球菌df101菌株中分离到隐秘的小质粒pefr ，全序列分析显示质粒pefr由3176bp组成，编码四个推定的orf ， orf1编码的一个推定的蛋白和复制有关的repa有很高的相似性。 |
| 10. | Phaa , phab and phac were inserted to pbv - 220 with double digest of restriction enduonuclease . the expression vectors of pbv - a , pbv - b and pbv - c were constructed by orientaional cloning . indefication of expression vector with restriction enduonuclease digest showed that phaa phab and phac were in right orfs
将phaa 、 phab和phac片段双酶切后，定向克隆至原核表达载体pbv220 ，构建了三个原核表达载体pbv - a 、 pbv - b和pbv - c ；经酶切分析表明，所克隆的三个基因phaa 、 phab和phac置于表达载体的正确阅读框架下。 |
