| 1. | Deletion of this part leads to loss of the gap activity completely . but this part is beyond the catalytic domain
有研究表明，在n末端己删除的基础上，继续删除第754位氨基酸，将导致rplgap失去全部gap活性。 |
| 2. | Hydrophobicity analysis of the noeb showed that it is a transmembrane protein and includes four transmembrane regions at n - terminal . , consisting of three primary helixs and one secondary helix
疏水性分析发现， noeb是一个跨膜蛋白，在n末端有4个跨膜区，其中包含3个初级螺旋和1个次级螺旋。 |
| 3. | The sequence encodes an open reading frame of 518 amino acids . there is a transit peptide of 74 amino acids in the n terminal of the putative amino acids sequence coded by the cdna
该序列长为1758hp ，起始密码子位于84 86hp ，终止密码子为1658 166fop ，开放阅读框长为1557hp ，编码58个氨基酸序列，其中n末端含有74个氨基酸的转运肽。 |
| 4. | Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115 . mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting
本研究将含有n末端信号肽和c末端毒性区缺失的pap基因的表达载体ppic9k - p用sali酶切线性化后，通过电击转化整合p . pastorisgs115菌株细胞中。 |
| 5. | A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein . the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis . it was demonstrated that constructed phagemid can still express penicillin g acylase
将包含信号肽和琥珀终止密码子uag ( amber )的完整巨大芽孢杆菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ，通过引入的11肽连接青霉素g酰化酶的c末端与噬菌体外壳蛋白gp3的n末端。 |
| 6. | - acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms . the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product . in this paper , - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation , heat treatment , deae - sepharose fast flow column chromatography
本文对来源于枯草芽孢杆菌( bacillussubtilis ) 3226 - 5的-乙酰乳酸脱羧酶经硫酸铵分级沉淀、热处理、 deae - sepharosefastflow离子交换柱层析等分离纯化步骤，得到sds - paeg电泳纯，通过n末端氨基酸序列分析验证酶蛋白的纯度。 |
| 7. | So it is important to reveal the mechanism of uptake and release of copper in hepatocyte , we aimed to purify a novel copper binding protein from rat liver lysosomal fraction . researches show that copper binding proteins have conserved amino acid sequences - mxcxxc ( x is any amino acid ) , can bind with copper . for example atp7b has five mxcxxc sequence , and it was determined 1 mol atp7b can interact 5 mol of copper ions
Irene等人的研究表明钢结合蛋白具有高度同源性的钢结合氨基酸序列，从酵母等低等动物到真核细胞n末端都有mxcxxcx代表任意氨基酸厂是钢结合的区域，例如ai ” ob的n末端就有5个mxc状c结构，实验也证明一摩尔的蛋白质可与5摩尔的硫酸铜结合。 |
| 8. | The results showed that the open reading frame of chil - 15 cdna encompassed 564 base pairs ( bp ) and encoded a protein of 187 amino acids with three potential n - linked glycosylation sites , four conserved cysteine residues , two out - of - frame atg initiation codons in the 5 " untranslated region , and a signal peptide consisting of 66 amino acids . when it was compared with the published sequence of chil - 15 cdna , 7 mutant sites were found , and 5 amino acids were changed in predicted amino acids , which indicated that chil - 15 may be polymorphic
结果显示，本研究所用白来航鸡il - 15cdna5 ’非编码区有两个框外atg起始密码子，开放阅读框由564bp组成，编码187个氨基酸，其中n末端信号肽含有66个氨基酸残基，在第48 、 149和166位的天冬酰胺残基上有三个潜在的n -糖基化位点。 |
| 9. | On the other hand , our previous studies have shown that the nr2b construct with a gfp tag in its n - terminus cotransfected with nr1 subunit can reach the cell surface and be detected by surface staining with anti - gfp antibody in live cells . the nr2b construct will be retained in the endoplasmic reticulum ( er ) when expressed alone in heterologous cells
我们以往的研究表明，用绿荧光蛋白( gf内在信号肤下游n末端标记nrzb亚单位，与nri共转染时能形成与野生型相似的nmda受体通道，且可以通过抗gfp抗体标记活细胞膜表面表达的nmda受体复合物。 |
