Guidelines for the conduct of tests for distinctness , uniformity and stability ramie boehmeria nivea l 植物新品种特异性一致性和稳定性测试指南苎麻
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Without a doubt , nivea has become a leading brand in the international skin - care market , and one of the most famous german companies in china ' s market Nivea已发展成为世界领先的护肤品牌，并成为中国市场上最知名的德国独资企业之一。
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Consequently it is very significant to develop transgenic plant vaccines . the regeneration system of boechtneria nivea l . guad was created in this research 因此选用合适的抗原，研制轮状病毒的转基因植物可饲疫苗具有重大的理论意义和实践意义。
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At the same time , vp4 gene was mutated in a certain point by pcr . the plant expression vector : pbi121 was constructed and then transformed to agrobacteriutn tumefaciens eha105 directly . a . tumefaciens eha105 harboring pbi121 / vp4 was used to transform the boechmeria nivea l . guad according to the leaf disc procedure 为便于基因操作，对外壳抗原蛋白vp4基因进行适当的修饰和改造：通过引物设计，利用pcr反应，在基因的起始编码前引入有助于真核生物表达的kozak序列和限制性内切酶位点；使用套叠pcr对vp4基因进行定点突变，以便于将改造后的基因插入pbi121构建植物表达载体，通过直接转化法，把pbi121 vp4转入农杆菌eha105 ，构建了农杆菌工程菌。
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Transformed shoots were selected on solidified medium containing kanamycin . ten kanamycin resistant transformants were obtained by direct or induction calla . these transformants were checked by pcr , pcr - southern blot and southern blot , confirmed that two positive transformants were integrated into the genome of boechmeria nivea l guad 对所得到的抗性转化株进行了pcr 、 pcr - southernblot 、 southernblot检测，其中2株呈阳性，证明vp4基因已整合到苎麻基因组dna中。
