| 1. | Megablast is one of the most important programs in ncbi blast basic local alignment search tool toolkits
序列比对是生物信息学研究中一个最基本的问题之一。 |
| 2. | Correction of five different types of errors of model refseqs appeared in ncbi human gene database only by using two novel human genes c17orf32 and znf
人类基因数据库模式参考序列5种错误类型的分析与纠正 |
| 3. | By ncbi - psi - blast search ( http : / / www . ncbi . rilm . nih . gov / blast / ) no protein with significant sequence similarity to pprl was found , suggesting that it is a unique gene in d . radiodurans
通过蛋白质序列同源性分析表明ppri较独特，在ncbi数据库中找不到较高同源性的蛋白。 |
| 4. | Two positive clones were obtained and sequenced . using the blast against ncbi gene database , they were comfirmed to be the cdna fragments of testin and actn1
筛选得到两个阳性克隆，测序后经blast软件比较基因库确定这两个阳性克隆中所含cdna分别为已知基因testin和actn1的片段。 |
| 5. | In the third part , we used m4g3 ascites as probe to screen the library for obtaining positive clone . once it was found , we would go on sequencing and analyzing at web ncbi / blast
第三部分：应用m4g3单克隆抗体筛选cdna文库，得到阳性克隆，并加以纯化，序列分析，通过internet互联网络使用ncbi blast程序库进行同源性检索。 |
| 6. | Using a set of primers shared by endogenous and exogenous alvs ( a > b > c , d , e ) , gp85 gene was amplified by polymerase chain reaction ( pcr ) from dna extracted from common cef and exogenous alv positive samples
根据已经发表在ncbi的alv - a 、 b 、 e亚群的序列情况，分析多态性酶切位点，运用不同的限制酶bgl ， bamh ， ssp进行分型。 |
| 7. | Its repetitive motif typically is ( ggp ) nggx and the homology with argiope trifasciata fiagelliform silk protein mrna ( atff ) is 69 . 3 % , which is the maximum comparing with all silk genes known in ncbi genebank
其典型重复结构是( ggp ) _ nggx 。与现有已知的蛛丝蛋白基因中三带金蛛( argiopetrifasciata )鞭毛样丝基因( atff )有最高的同源性69 . 3 。 |
| 8. | After a comparison of all the ghrs isolated from the present study and others available at ncbi database , we find that ghr2 only exist in teleosts , and there is no such a gene in other vertebrates
在得到以上ghr序列后，又分析了人、鼠、鸡等其他动物的基因组，发现ghr2只存在于鱼类，是鱼类特有的现象。本研究对ghr1和ghr2做了详细的比较分析。 |
| 9. | The high homology genes are not found in all genes known . but the putative conserved domains of peptidase have been detected . in the domains the homology comparing with callus gallus cathepsin b cdna is 90 %
Ncbi检索未见同源性高的基因序列，但发现含有典型的肽酶结构域，编码蛋白与组织蛋白酶b结构域部分一致性高达90 ，其具体功能有待于进一步验证。 |
| 10. | Ncbi blastn analysis indicated that similarity of hsa cdna sequence and the postulated ammonic acids sequence is 99 . 6 % and 99 . 5 % , compared with the reported sequence in genbank . so the modified hsa cdna with kozak sequence was obtained
Ncbiblastn分析的结果表明，获得的hsacdna序列及推导的氨基酸序列与genbank中所报道的序列的同源率分别为99 . 6 、 99 . 5 。 |
