| 1. | In this study , we found that enucleated maturation oocytes of bovine and mouse can only support nuclear transfer embryos to develop to morula
60o和8 50o的桑堪胚发育；重组胚胎在mi99和改良的czb中培养，分别获得16 41o和17 |
| 2. | 6 - dmap + ccb + ethanol can effectively activate the reconstructed rat - mouse embryos . 5 ) 225 reconstructed embryos from 2 - cell to morula were transferred into 46 recipients
23 l6 dmap乙醇ccb能有效地激活种间核移植重构胚。 |
| 3. | The fresh mouse morulae were bisected following decompaction ( experiment 3 ) and other morulae without decompaction ( experiment 4 ) also splitted
新鲜桑椹胚经去致密化处理后分割，其分割成功率、半胚培养后的囊胚发育率都显著高于未处理组。 |
| 4. | The results show that the development rates of morula treated with serum starvation for 3d , 4d , 5d are higher apparently than nocodazole group and control group ( p < 0 . 05 )
直接注射法构建的核移植重组胚胎的桑堪胚发育率（ 25 95 ）高于电融合法（ 16 07 ） ，统计学分析差异显著（ p 0 |
| 5. | Two stage embryos were observed their effect on production of chimera . the result shows that there is a significant different ( 0 . 01 < p < 0 . 05 ) between blastocysts ( including early blastocysts and morulae ) and expanded blastocysts
结果表明，囊胚、早囊及桑椹胚同扩张囊胚两种注射胚胎制作嵌合体所产生的结果差异显著( 0 . 01 p 0 . 05 ) 。 |
| 6. | 4 ) after the activated nuclear transfer embryos had been cultured in m199 ^ and czbm for 48h , the cleavage rate can reach at 16 . 41 % and 17 . 41 % , and the morula rate were 7 . 46 % , % and 6 . 74 % , respectively
08融合率：血请饥饿法和nocodazole法处理的供体细胞构建的重组胚胎，经6 dmap乙醇ccb激活，分别获得17 33o和17 02o的卵裂率以及6 |
| 7. | Experiment i study on the embryonic development by injecting different developmental stage embryos . this experiment collected 109 embryos to observe their developmental ability by es cells microinjection into 8 - 16 cell stage morula or blastocyst
本试验先后将109枚胚胎用于此项研究，通过将小鼠es细胞注入8 - 16细胞桑椹胚和囊胚，观察两个不同时期注射胚胎的发育能力。 |
| 8. | Ppar5 expression in the uterus of ovariectomized mice was significantly stimulated by estrogen or a combination of estrogen and progesterone . ppar8 mrna was detected in oocytes and preimplantation embryos at the stages of zygote , 2 - cell , 4 - cell , 8 - cell , morula and blastocyst
在小鼠卵母细胞、受精卵、 2细胞、桑椹胚和胚泡中，可检测到ppar表达，但在4细胞和8细胞中没有检测到ppar基因的表达。 |
| 9. | In the 2 - step ops method , embryos were pretreated with 10 % eg + 10 % dmso for 30s before exposed to edfs30 , a vitrification solution , for 25s , then immersed in liquid nitrogen . the blastocyst rate of vitrified mouse morula after equilibration in 0 . 5mol / l sucrose for 5min was 100 %
Ops二步法即胚胎预处理30s ，然后移入edfs30冷冻液中平衡25s冷冻保存，解冻后在0 . 5mol l蔗糖溶液中平衡5min ，培养后囊胚发育率为( 100 ) 。 |
| 10. | The demi - embryos derived from vitrified morulae in ops ( experiment 7 ) and fresh morulae ( experiment 8 ) after cultivation for 24h were transferred to day 2 . 5 to day 3 pseudo - pregnant female mice . in experiment 7 , 15 fetuses were obtained from 5 of 11 recipients which had received 191 demi - embryos
对ops法冷冻桑椹胚和新鲜桑椹胚分割后移植，将各自分割所获得的半胚191枚和182枚，分别移植给11只和10只假妊娠2 . 5 3d的受体母鼠。 |
