The subject in this study is the recombination , expression , purification and biological activity on antibacterial fusion peptide of , magainin - protegrin 本实验主要研究的是magainin - protegrin杂合抗菌肽基因工程菌的构建、表达、纯化及活性测定。
2.
Firstly , the amino acid sequence of major bioactivity hi magainin and pro - tegrinin have been founded from the genebank and then the related gene has been artifically synthesized . the enzyme sites are added to the two sides of the synthesized gene 该实验通过取magainin和protegrin主要生物活性的氨基酸序列并人工合成其编码基因,在合成基因的两侧添加适当的酶切位点。
3.
Magainin is the skin secretion product of batrachia under the eriviroment pressure , which can be used to accelerate the fusion rate of wound and resist the infection . it can inhibit the growth of bacteria and fungus with the lowest concentration ; protegrin is isolated from porcine leukocyte hi 1993 and is useful as antiviral agents Magainins是爪蟾的皮肤在一定的环境压力下分泌出的抗感染和促进伤口愈合的成分,低浓度便可抑制许多细菌和真菌生长; protegrin是1993年从猪的中性白细胞中分离得到,研究发现它具有抗爱滋病毒的功能。