| 1. | The harmfulness of l . lagurus is a serious problem for long time , because of undulation of population number and perniciousness
由于其种群数量变动较大，加之它对草场的危害严重，长期以来一直困扰着人们。 |
| 2. | After rt - pcr of total rna isolated from the ovaries of lagurus lagurus , the lzp3 cdna was cloned into pucm - t and further identified with sequence analysis
将扩增产物连接到pmd18 - t载体上进行测序，序列分析表明已克隆出lzp3全长cdna 。 |
| 3. | Extensive mitochondrial dna polymorphisms were found among lagurus lagurus , mus musculu ， rattus norvegicus and mice . the findings will help us to understand the dispersion and evolution of these animals
通过琼脂糖凝胶电泳对这些片段进行测定，同时估算出草原兔尾鼠线粒体dna的长度约为16 . 6kb 。 |
| 4. | As one of the most promising population control tools in the world today , immuno - contraception using zona pellucida glycoprotein antigens , specifically zp3 , may be used to control lagurus population
卵透明带3 （ zp3 ）蛋白是哺乳动物生殖过程中介导精卵结合的关键因子，已被用作免疫不育控制鼠害研究的主要靶抗原。 |
| 5. | With a pair of degenerate primers designed according to the conserved region among the known cdna sequences of five rodents , a cdna fragment was generated by rt - pcr from total rna isolated from ovaries of lagurus lagurus
首先根据已发表的五种啮齿目动物zp3cdna序列同源性分析结果设计特异性引物， rt - pcr扩增草原兔尾鼠zp3 （ lzp3 ） cdna保守序列。 |
| 6. | In this study , by combining rt - pcr and race , a full - length cdna encoding the lagurus lagurus zp3 ( lzp3 ) was isolated from the lagurus ' s ovary and cloned into a prokaryotic expression vector and a eukaryotic expression vector for further immunocontraceptive investigations
本论文研究工作采用rt - pcr与race相结合的方法，首次克隆了草原兔尾鼠zp3全长cdna并进行了序列分析，构建了真核表达质粒，为草原兔尾鼠免疫不育的研究奠定了基础。 |
| 7. | According to measured data , testis sizes from animals captured in september 2000 were different remarkably from that in april and july 2001 , and not differences among their age groups , which was indicated that l . lagurus in september 2000 did not have reproductive abilities . by use the same method , we have observed that l . lagurus in april 2001 and groups from - age and - age ( old ) in july 2001 have reproductive abilities . in addition , the reproductive ability from the - age group in july 2001 was intermediated between the groups in september 2000 , - age group in april and - age group in july 2001
我们对不同时间参与繁殖的雄鼠睾丸大小的测定结果表明， 2000年9月雄鼠睾丸大小与2001年4月和7月的有显著性差异，表明2000年9月草原兔尾鼠雄鼠进入繁殖休止期； 2001年4月种群中龄和龄雄鼠睾丸大小无显著性差异，与2001年7月的相比有显著性差异，但与2001年7月种群的龄鼠和龄鼠的相比无显著性差异，表明2001年4月和2001年7月种群的龄和龄草原兔尾鼠雄鼠具繁殖能力； 2001年7月的龄雄鼠睾丸大小与2000年9月的有显著性差异，表明该时期龄雄鼠的繁殖力介于2000年9月种群和2001年4月和7月种群的龄鼠和龄鼠之间。 |
| 8. | According to the live coefficient c , we modified the predictions for this age group and come to a conclusion that is corresponding to actual field data . in other words , the numbers of - age and - age were nearly zero with their densities below 4 / hm2 . these results suggest that l . lagurus population began to decline
运用校正后的存活系数继续预测，其结果与2001年9月的野外调查结果基本一致，证实当年草原兔尾鼠的数量非常少，低于4 . 0只/公顷，且龄鼠和龄鼠几乎为零。 |
| 9. | In addition , for construction of prototype dna vaccine , pcmv4 - lzp3 , the cloned lzp3 full - length cdna was subcloned into a eukaryotic expression vector , pcmv4 . these preliminary results could lead to further investigation for the development of immunocontraceptive vaccine based on lzp3 for lagurus population control
本工作首次克隆了草原兔尾鼠卵透明带3全长cdna ，已向genebank申请注册为新基因，保护了我国的野生动物基因资源，为哺乳动物生殖机理的研究提供了新的资料。 |
| 10. | With a set of primers designed according to the sequence of the cloned cdna fragment , the 3 " and 5 " ends of the cdnas were obtained by 5 " and 3 " rapid amplification of cdna ends ( race ) , respectively . combining the sequences of the 3 " ends , 5 " ends and cloned partial cdna , the full - length sequence of the lagurus lagurus zp3 ( lzp3 ) cdna was assembled
根据已测序的lzp3保守序列设计特异性引物，使用3 '和5 ' race的方法扩增lzp3cdna的3 '末端和5 '末端序列并进行测序，所测序列经与lzp3保守序列相互重叠、核对与拼接，获得了lzp3全长cdna序列。 |
