This group of mutant alleles turned out to be a gene encoding a golgi adenosine 3 ' - phosphate 5 ' - phosphsulfate transporter ( adenosine paps transporter ) . kyte - doolittle hydrophilicity plots showed that sll is a very hydrophobic protein , especially in c terminus Kyte - doolittle疏水性分析结果表明，与其它转运子相似， sll蛋白是一个疏水性很强的蛋白(尤其在它的c端) 。
2.
Main methods and results are as followed : 1 epitope analysis of agonist - binding region of nrla physicochemical properties and antigenicity of two agonist - binding regions of nrla were analyzed through bioinformatics : domain p1 containing 151 amino acid residues preceding the first transmembrane domain of the human nrla , domain p2 with 144 residues following the third transmembrane domain . four parameters including hopp - woods and kyte hydrophilicityjanin accessibility , karplus - schulz flexibility , and welling antigenicity were used to determine the antigenic sites , and prosite programme and chou - fasman method were employed to analyze their related sequence motif and the secondary structures 用goldkey软件分别选取公认的hopp等与kyte等亲水性参数、 jain表面可及性参数、 karplus - schulz主链柔韧性参数及welling抗原性参数对p1 、 p2两个多肽片段进行参数分析。并采用通用的prosite程序与chou - fasman方法比较分析p1 、 p2多肽片段的氨基酸位点与二级结构特征。综合判定两个多肽片段的抗原性及其位点，结果认为p2抗原性强于p1 。
