| 1. | Effect of cysteamine on the plasma contents of gh , ss and igf - i in adult geese
半胱胺对鹅生长内分泌的影响 |
| 2. | Igf - i , insulin - like growth factor
胰岛素样的生长激素 |
| 3. | The e . coli bl21 ( de3 ) was transformed with the constructed recombinant plasmid pexsecl - igf - i
已构建好的含重组质粒pexseci - igf - i的e |
| 4. | We modified it with a synthetic gene that would produce igf - i only in skeletal muscle and began by trying it out in normal mice
我们将一段只会在骨骼肌内制造igf - 1的基因加进病毒，然后利用一般的小鼠来测试。 |
| 5. | So it is very helpful to advance the research and application of igf - i to establish a relatively simple proficient efficient purification route
所以建立一条相对简单、高效的纯化路线，有利于推动igf - i研究和应用。 |
| 6. | We find that the fusion proteins are inclusion bodies when the bacteria lysised by the lysozyme . the product of pet - 32a ( + ) - igf - i was analyzed by western blotting , which confirmed that the hybrid protein was expressed as expected
菌体采用溶菌酶裂解，融合蛋白以包涵体形式存在；对higf -融合蛋白进行了westernblot分析，确认所表达蛋白为目的蛋白。 |
| 7. | Insulin - like growth factor - i ( igf - i ) is a kind of active peptide that can promote many types of cells to grow . it plays an important regulatory role in the proliferation , differentiation , apoptosis of tissue cell and tumorigeneisis
胰岛素样生长因子- i ( insulin - likegrowthfactor - i ， igf - i )是一种具有促进多种细胞生长功能的活性多肽，它对组织细胞的增殖、分化、凋亡，机体的生长发育及肿瘤的发生发展起重要的调节作用。 |
| 8. | Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site , and the igf - i product of pcr contains 230 base pairs . igf - ii contains 219 base pairs . 3
各另外设计一对特异性pcr引物，导入适当限制性内切酶切点，以上述连有目的基因的克隆载体为模板，采用pcr方法扩增基因片段，获得长度约230bp的igf -和219bp的igf -成熟肽基因序列。 |
| 9. | Research , japan . there dr . jing helped to set up a primary culture system of neuroepithelial cells of day 10 mouse embryos . then he found a human teratocarcinoma cell line , pa - 1 , secreted the growth factors related to egf and igf - i
曾于1989 1991年赴日本理化学研究所进行博士后研究；于1992 1993年以访问学者身份赴日本理化学研究所进行合作研究； 1995年以访问学者身份在法国strasbourg的遗传与分子细胞生物学研究所( gbmc )进行合 |
| 10. | Sds - page analysis suggested that the bacteria containing the recombinant plasmid pet - 32a ( + ) - igf - i produced the fusion protein of 30kda as it was induced by iptg . consisting 10 % of the total bacterial proteins , and the pet - 30a ( + ) - igf - ii produced the fusion protein of 14kda , which consisting 35 % of total bacterial proteins . 5
Sds - page分析表明，重组质粒pet - 32a ( + ) - igf -在iptg诱导下表达分子量约30kda的融合蛋白，但其表达量不高，约为菌体总蛋白的10左右；重组质粒pet - 30a ( + ) - igf -在iptg诱导下表达分子量约14kda的重组蛋白，融合蛋白表达量约占菌体蛋白总量的35 。 |
