Performance of the chinese hamster ovary cell hypoxanthine guanine phosphoribosyl transferase gene mutation assay 中国仓鼠卵巢细胞次黄质鸟嘌呤转磷酸核糖基酶基因变异鉴定的操作
2.
Few studies have been conducted so far on meiotic progression and none was carried out on its control via hypoxanthine in goat oocytes 但是，有关山羊卵母细胞的减数分裂进程及其调控均有待进一步的研究。
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In this study , the meiotic progression and its control by hypoxanthine were studied in the goat oocytes aspirated from slaughtered ovaries 本实验以随机屠宰山羊卵巢为材料研究了山羊卵母细胞减数分裂的进程以及hx在这一过程中的调控作用。
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2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6 . their antibodies " isotypes are tested by goad anti - mouse isotype regent 取驯化好并处于对数生长期的2 - e _ 4 - a和s _ 2 - b细胞，再常规融合和筛选，三次克隆化后得稳定分泌双特异性抗体的杂交-杂交瘤细胞株6株。
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Abstract : the early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro . many studies of phosphorus , glucose , hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology , biochemistry , molecular biology and micromanipulation have been reviewed . the merit and shortcoming were analyzed and the necessity of using simple or components limited media overcoming early embryo developmental block were also reviewed . media that have been shown effective in overcoming early embryo developmental block in mouse , rat , hamster , rabbit , pig , sheep , cattle and monkey were listed 摘要哺乳动物胚胎在体外培养中普遍存在早期发育阻滞的现象.对此,人们用形态学、生物化学、分子生物学、显微操作等手段开展了磷酸、葡萄糖、次黄嘌呤和细胞质因素对早期胚胎发育阻滞的影响的研究.本文综合分析了共培养系统的优缺点.说明了采用完全成分已知的培养液对进行有关研究的必要性.列出了有效运用于克服小鼠、大鼠、仓鼠、兔、猪、羊、牛、猴等动物早期胚胎阻滞的成分已知的培养液的名称。
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Rat cumulus - enclosed oocyte ( ceo ) and denuded oocyte ( do ) both spontaneously resume meiotic maturation in vitro , when cultured for 2h , the germinal vesical breakdown percentage of do reaches up to 88 % , and ceos get 53 % . oocytes begin to extrude first polar body 1 ( fb1 ) when cultured for 8h . mouse oocyte spontaneous maturation in vitro can be inhibited by 4mm hypoxanthine ( hx ) or 25um 3 - isobutyl - methylxanthine ( ibmx ) . while , 4mm hx can not inhibit the spontaneous maturation of rat oocyte , and the lowest effective concentration of ibmx to inhibit rat oocyle spontaneous maturation in vitro is 50um . treated with the same concentration of ibmx , gvbd percentage of rat ceo is lower than that of do , the results indicat that cumulus cells delay rat oocyte spontaneous maturation and potentate the inhibition effect of ibmx , but it ' s not the case on mouse Ceo和do培养8h时都开始排出pb1 ；在卵母细胞成熟抑制模型中， 4mm次黄嘌呤( hx )和25 m的异丁基甲基黄嘌呤( ibmx )是小鼠卵母细胞成熟的有效抑制剂，但4mm的hx抑制大鼠卵母细胞成熟效果不理想，用hx - m199和hx - mem培养24h ， gvbd率分别为61 ， 81 ，而ibmx有效抑制大鼠卵母细胞自发成熟的最小浓度为50 m ， ibmx可以降低大鼠ceo和do的gvbd率，但ceo中的卵母细胞gvbd率要显著低于do的gvbd率， 100 m的jbmx处理24h后，大鼠ceo和do的gvbd率分别为24 ， 84 ，提示卵丘细胞的存在可以延迟大鼠卵母细胞的自发成熟，有助于抑制卵母细胞成熟，但在小鼠上ceo和do的自发成熟差异不明显。
