English translation for "htb"

华天牌

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Example Sentences:

	1.	The gene was subcloned into the pproextm htb vector , then was transformed the vector into e . coli dh5 . a biologically active recombinant pil - 10 was obtained after induction by iptg 然后将pil - 10编码基因亚克隆到表达载体pproextmhtb中，转化dh5后，用iptg诱导培养，表达了具有活性的重组pil - 10 。
	2.	Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538 , and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid . both vp3 gene and lacz gene were cloned into noti site of psy681 , recombinan fpv transfer vector containing vp3 gene of gpv was obtained . the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine 本研究另从含有gpvh1分离株主要结构蛋白vp3基因的重组质粒pproexhtb - vp3中切取gpvh1株vp3基因片段，将其亚克隆于psy538的ecori位点，然后将带有痘苗病毒启动子p11的lacz报告基因平端克隆于上述重组子的smai位点，再用noti切下同时含有vp3基因和lacz报告基因的片段，再亚克隆于psy681的noti位点，构建出含有vp3基因的重组禽痘病毒转移载体，为构建表达vp3基因的重组禽痘病毒从而制备gpv基因工程疫苗奠定了基础。
	3.	By recombinant dna techniques , the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb . the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e . coli dh5a and induced with iptg . sds - page analysis showed an induced expression product band about 72ku , which correspond to the sizes of vp2 , reported in the literature 利用dna重组技术，将其结构蛋白vp2基因亚克隆至原核表达载体pproex - htb ， iptg诱导后成功表达出与预期大小相符的约72ku的融合蛋白，光密度扫描对表达产物进行初步定量，表明表达产物约占菌体总蛋白的14 。
	4.	The expression conditions of e2 gene in p . pastoris were optimized , the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7 . 5 and 8 . 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e . coli the expression vector pproex - htb respectively , the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e . coli competent bacteria respectively , the recombinant bacteria could express the major antigen region of e2 gene , the expression yields amount to 35 % and 38 % repectively 猪瘟病毒ez基因的原核表达： pcr扩增出当前猪瘟流行野毒株，中国猪瘟兔化弱毒（ c株）兔脾组织毒ez基因的主要抗原区，将其克隆到原核大肠杆菌表达载体pproex htb中诱导表达，经sds page检测表明，重组质粒能表达ez基因主要区蛋白， westernblot检测表明，诱导表达蛋白与猪瘟阳性血清发生特异性反应，表达量为35和38 ，可用于基因工程诊断抗原。

Similar Words：: "htat" English translation, "htaungyan" English translation, "htaw" English translation, "htawhpan" English translation, "htay" English translation, "htb hexadecimal to binary" English translation, "htb-205" English translation, "htbhyper text broadcasting protocol" English translation, "htbm" English translation, "htc" English translation