| 1. | Therefore hela cells provide a good model for the study the mechbosm of llp - hsdl gene expression by
将克隆的含gre样结构的0叫基因启动子1 |
| 2. | It has been demonstraed that there are differential distributions of l 1 p - hsdl and 11 p - hsd2 in the fetal membranes and placenta
在体内， pghs存在两型，其中pghs - 1为细胞固有型， pghs - 2为可诱导型，细胞因子、激素及毒素等上调其表达。 |
| 3. | Sdring for llp - hsdl revealed a cytoplasinic distribution in the chorionic tiophoblast . twunoreactive gr mg was found both in the cy ' toplasm and nucieus of the cell
11p hsdi主要分布于胞浆中， gr虽然在胞核和胞浆均有分布，但也以胞浆分布为主 |
| 4. | However , whether glucocothcoids modulate llp - hsd1 expression through a prgnoter dependent mechanism has never been studied . in this study we bo exainined whether 1l9 - hsdl and gr are co - expressed in the sarne chorionic trophoblast
胎盘中两型酶均存在， 11 - hsd1主要存在于胎膜的绒毛膜滋养层细胞，而11 - hsd2主要存在于胎盘的合体滋养层细胞。 |
| 5. | 3 . ewe radiometric conversion assay showed that hela cells have the reductase activity of ll9 - hsdl , which suggests there is the molecular system for llp - hsdl expression in hela cells . and other reports showed hela cells express gr
利用tlc方法证实人类子宫癌细胞株hela具有11po酶活性，而且文献报道hela细胞株表达gr ，说明hela细胞株是研究gc调节11p0机制比较的理想模型 |
| 6. | Which may provide the molecular basis for the inberine regulation of ll p - hsdl expression by glucocorticoids . secondiy , the regulation of l l 9 - hsdl expression and redutase activity by glucocorticoids was investigated in the primny cultured human chorionic trophoblast
整个妊娠过程中胎盘11 - hsd活性以氧化酶为主，使母体gc在进入胎儿循环前大部分被转化为无活性的17 -羟- 11 -脱氢皮质酮，保证胎儿正常发育所需的激素浓度。 |
| 7. | L lp - hsdl ma of i . 5kb was detected in the rna extracted from cult ' ured chorionic trophoblast using northem blot hybridhation method . the expression of ll6 - hsdl wa was induced by treating the chorionic cells with dexamethasone ( l0 - ' , l0 ' m ) for 24h , and the induction was blocked by co - treatinent of the cells with ru486 . these results suggest a gr - mediated effect of glucocorticoid on the rnrna expression and reductase activity of l l 9 - hsd1
人h合成的肌?一加x门’ m ）对培养的绒毛膜滋养层细胞的11p hsdi还原酶活性具有诱导作用，此诱导作用可以被gr阻断剂ru486门0川）所阻断； northern印迹杂交方法进一步证实， dex亦可诱导绒毛膜滋养层细胞11p叫inrna的表达，此诱导作用也可以被ru486门0叫川）所阻断 |
| 8. | The co - expression of l l6 - hsd1 and gr in the saxne chorionic trophoblast suggests possible intrcrine achons ofglucoconicoid generated by l l6 - hsdl within the cells . 2 . ewe radiomctric conversion assay showed that trctrient of the cells with the synthetic glucocorticoid - - dexarnethasone ( l0 - ' m ) for 24h increased the conversion of conisone to cortisol , and thes increase was blocked by the gr antagonist ru486
双标免疫组织化学染色结果显示11p hsdi和gr共存于同一个滋养层细胞，提示在体内无活性的gc代谢产物?一17羟d脱氢皮质酮经11p hsdi还原活化后可以直接与同一个细胞内的gr结合，即以内在分泌（ intracrine ）形式发挥作用 |
| 9. | The full length cdnas of 17 - hsd1 and 17 - hsd3 , 17 - hsd8 were obtained by library screening and race , respectively . expression patterns ( tissue distribution ) of three types 17 - hsds were checked by rt - pcr and northern blot . the recombinant constructs of 17 - hsdl / pet blue2 and 17 - hsd8 / pcdna 3 . 1 were made and subsequently transformed into the corresponding host expression cell of ( de3 ) placi and mammalian hek 293 cell
首先从genebank下载在其他脊椎动物中已被克隆17 - hsd1 ， 17 - hsd3的氨基酸和核甘酸序列，并在序列保守区域设计简并引物，然后分别以罗非鱼卵巢和精巢cdna为模板进行rt - pcr扩增得到17 - hsd1 ， 17 - hsd3的中间片段。 |
| 10. | L l6 - hsd2 is localized to the sppytiotrophoblast of the placenty providing a fimctional barrier protecting the fetus from matemal glucocorticoids . a sequence resembling glucocorticoid response element ( gre ) has been identified in the promoter region of the human l l0 - hsdl gene . glucocorticoids have been shown to induce the expression of 11p - hsdl in the hippomus in vitro " whereas controversial results were obtained in the hepatocyte
体内至少存在两型11 - hsd ，在细胞完整的状态下， 11 - hsd1主要为还原酶，它活化gc的代谢产物17 -羟- 11 -脱氢皮质酮(啮齿类为脱氢皮质酮)为有活性的皮质醇(啮齿类为皮质酮) ，而11 - hsd2为氧化酶，它催化皮质醇为无活性的17 -羟- 11 -脱氢皮质酮，因此11 - hsd1加强gc的作用，而11 - hsd2减弱gc的作用。 |
