| 1. | 6 . the dna sequence was assayed to determine whether the sequence of er ? was we wanted
Dna序列测定，鉴定hela细胞中er6的pcr产物是否为所需序列： 7 |
| 2. | Using the superfect reagent , in vitro cultured hela cells were introduced with external plasmids
利用脂质体superfect介导的转染方法将外源质粒导入体外培养的hela细胞。 |
| 3. | The culture of hela cervical carcinoma cell line and bcap - 37 breast carcinoma cell line
宫颈癌hela细胞系和乳腺癌bcap上7细胞系传代培养； ihp wgz第四军医大学硕士学位论文3 |
| 4. | ( 2 ) effects of the expression of telomerase rna gene on the telomerase activity in human normal peripheral blood leukocytes , cell growth and cell proliferation
利用基因工程技术从hela细胞中克隆了人端粒酶rna组分( htr )基因。 |
| 5. | The shrna based on telomerase htert gene 1573 - 1591 sequence can silence hela cell telomerase gene expression in a detectable extent
以端粒酶tert基因1573 ? 1591位的核酸序列构建的shrna可对hela细胞端粒酶基因表达产生一定的沉寂作用。 |
| 6. | With different amount shrnas transfected , in different time , we assayed the telomerase activity of the transfected cells with trap - silver staining and pcr - eia methods
用trap -银染法和pcr - eia分别检测经转染不同shrna量和作用时间hela细胞端粒酶活性。 |
| 7. | First , the expression of cd55 , cd59 on human t cell line mt4 , the host cell of hiv , and on human epithelia hela cell , the host cell of eev was detected with facs
为此，本研究分别以人t细胞系mt _ 4细胞及人上皮细胞hela细胞为宿主细胞，制备收集了hiv ? b病毒及eev病毒。 |
| 8. | After transfecting the shrna based on telomerase htert into hela cells with calcium phosphate co - precipitation procedures , we detected hela cells viability by trpan blue staining method
以磷酸钙共沉淀转染法将shrna转染hela细胞。于不同的时间用台盼蓝染色法检测细胞生存能力。 |
| 9. | With the polyclonal antiserum against testin and actn1 , and the monoclonal antibody against ap - 2a , the expression of testin , actn1 and ap - 2a in hela cell was determined
用制备所得的抗gst - testin血清和抗gst - actn血清及ap - 2的单克隆抗体分别检测了这三种蛋白质在hela细胞中的表达。 |
| 10. | Rbp - j directly interacts with ramic to activate transcription of downstream target genes that prevent cell differentiation . thus , rbp - j is a critical effector molecule in notch signaling pathway
5dpc胚胎和人的hela细胞cdna文库中筛选到一个可以与rbp - j第四军医大学博士学位论文相互作用的新蛋白kyot |
