As a kind of important intermediates , glycosyl isothiocyanates have been applied in the preparation of new compounds possessing considerable bioactivities 作为一类重要的有机合成中间体，糖基异硫氰酸酯广泛地应用于具有生理活性化合物的制备。
2.
As a kind of important intermediates , glycosyl isothiocyanates have been applied in the preparation of new compounds possessing considerable bioactivities 摘要作为一类重要的有机合成中间体，糖基异硫氰酸酯广泛地应用于具有生理活性化合物的制备。
3.
In this paper , the structures and the synthesis methods of glycosyl isothiocyanates are summarized , including the application as intermediates in the preparation of glycosylthioureas , glycosylheterocycles , glycosylguanidins and glycosylconjugates and so on 本文对糖基异硫氰酸酯的结构、制备方法，以及作为中间体在糖基硫脲、糖基杂环、胍基糖苷、糖基缀合物等重要化合物的合成中的应用作了较详尽阐述。
4.
Northern blot results show that nos . 66 - 1 , 84 , 89 - 1 , 97 , 108 , 152 , 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no . 23 has weak signal only in sp6 - tester , nos . 94 , 165 , 172 , 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos . 4 , 17 , 18 , 28 , 6 9 , 101 , 156 - 1 , 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no . 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76 , and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180 . 1 , supported by cdna : 7119 , supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no . 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685 , gi | 2605931 | gb | af029898 . 1 | af029898 ) for aspartate aminotransferase ; no . 89 - 1 cdna has significant alignment with arabidopsis tha 与同源性最高的拟南芥类似晚期胚胎发生高丰度蛋白比较，二者都具有lea 2结构域、保守分泌蛋白cog5608结构域和低复杂度区，都具有pkc磷酸化位点、酪蛋白激酶磷酸化位点、 n十四酞化位点和酚胺化位点，所不同的是： （ ）在结构功能域上， 152全长cdna编码的蛋白质序列中多了1个lea 2结构域、 l个保守分泌蛋白cog5608结构域和1个低复杂度区； （ 2 ）在功能位点上， 152全长cdna编码的蛋白质具有酪氨酸硫酸化位点、多了l个酪氨酸激酶磷酸化位点和1个可能的天冬氨酸富集区，但没有n糖基化位点； （ 3 ）拟南芥类似晚期胚胎发生高丰度蛋白的lea 2结构域具有显著性（ e
5.
Levels of fasting blood glucose and 24h urinary microcontent of albumin 24 h malb were determined dynamically ; the serum glycosyl hemoglobin hba1c was determined after the last medication ; the ultrastructural changes of kidney were observed by transmission electron microscope ; the expressions of collagen , fibronctin , laminin ln , and the ecm metabolism influencing factors , including mmp2 , tissue inhibitor of metalloproteinase timp2 , transfer growth factor 1 tgf 1 in renal tissue were detected by immunohistological chemistry and image collecting analytical system 动态检测各组大鼠空腹血糖fbg 24h尿微量白蛋白24h malb ，末次给药后测定大鼠血浆糖化血红蛋白hba1c透射电镜观察各组大鼠肾脏超微结构改变，应用免疫组化技术及图像采集分析系统测定各组大鼠肾脏组织中型胶原c纤维连接蛋白fn层粘连蛋白ln的表达，测定影响ecm代谢的基质金属蛋白酶2 mmp2基质金属蛋白酶抑制剂2 timp2及转化生长因子1 tgf 1的表达。
