Optimization on production conditions of fibrinolysin by bacillus subtilis sy 豆豉纤溶酶产生菌的产酶条件优化
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The influence of fibrinolysin and shuxuening injection on neuro - endocrine hormones of acute cerebral embolism 降纤酶合舒血宁注射液对脑梗死急性期神经内分泌激素的影响
3.
The 600 bp and 800 bp pcr products were cloned into the pgem - t easy vector . their cdna sequences were determined with positive clones or purified pcr product . conclusion : compared the 600 bp pcr product with the amino acids sequence for the fibrinolysin metalloproteinase from the venom of agkistrodon acutus from the southern of anhui province , their homology is 90 . 6 % 结果:其中一对引物扩增得到一600bp产物;另一对引物扩增得到三条特异性的dna条带,大小分别为1 . 5kb 、 1 . 3kb和800bp ,将600bp和800bpdna进行克隆及测序,并推导编码的氨基酸序列。