High frequency of shoot differentiation was obtained from the protocalli on differentiated medium . the regenerated plants still preserved resistance to methionine and ethionine 原生质体再生的植株仍然保持对甲硫氨酸的抗性，同时对乙硫氨酸表现交叉抗性。
2.
Somatic hybrid cells were obtained between ethionine resistant cell line of astragalus adsurgens pall , and agrobacterium / " / z / zogerae . s ' - transformed cell line of medicago saliva l . using protoplast fusion by peg method 通过peg诱导原生质体融合和杂种细胞的筛选培养，首次得到沙打旺( + )紫花苜蓿的属间体细胞杂种。
