Medical microbiology ; dyes ; xanthene dye , fluorescein derivate eosin y 医用微生物学.颜料.第2部分:咕砘颜料荧光素衍生物
3.
Following laser exposure , the samples were fixed in 4 % glutaraldehyde and sent for histologic study using eosin - staining techniques for subsequent analysis with light microscopy 在激光照射之后,样本固定在4的戊二醛中,曙红染色后用光显微镜分析进行组织学研究。
4.
In order to understand the mechanism of spermatozoa living in the spermatheca after copulation , hematoxylin - eosin dyeing method is used to discover the microstructure of the spermatheca by light microscope 为进一步了解雌雄个体交配后精子在受精囊内的存活的机制,采用h - e法,在光镜水平上研究了受精囊的显微结构。
5.
In this paper , the methods that the author used are as follows : light microscopy : the testis was fixed in bouin ' s fluid , dehydrated in an ethyl alcohol series , embedded in paraffin , sectioned at 6 u m and stained with hematoxylin and eosin , then observed with olympus microscopy and photographed 光镜样品以bouin ' s固定液固定,系列酒精脱水,石蜡包埋,切片厚度6 m ,苏木精、伊红染色, olympus显微镜观察并拍照。
6.
In the first trial , combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis , and then a modified discontinuous percoll gradient centrifugation method ( 15 % , 22 % , 30 % , 40 % , 50 % , 60 % ) was introduced to isolate spermatids from the cellular suspensions . the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis , and the viability of spermatogenic cells was assessed by using eosin y exclusion test 在第一部分试验中,首先利用连续3次组合酶消化成年小鼠睾丸制备睾丸细胞悬液,然后经6层非连续percoll梯度离心法( 15 、 22 、 30 、 40 、 50和60 )分离,通过形态学和流式细胞术鉴定南京医科大学硕士学位论文各个percoll组分中精子细胞的含量,并以伊红y排斥试验测定细胞的存活率。
7.
The early embryo were made into a series of continuous section slides by tissue cutting . the sections were stained by hematoxylin and eosin ( h & e ) staining and then the development of internal organs such as heart in early embryos was observed by microscope . we found that there is certain relationship between external and internal malformation 同时我们收集人类药物流产的早期胚胎,观察发现胚胎畸形占17 . 86 % ,早期致死占32 . 54 % ;采用组织切片技术将胚胎制成一系列石蜡连续切片,染色后显微观察畸形和正常的早期胚胎内部心脏等器官的发育情况,发现胚胎外部畸形与体内畸形存在一定关联,对此我们将做进一步的研究。