| 1. | It was thought to be a new gene encoding for chitinanse of b . subtilis . genome library methodology was adopted for cloning of endoglucanase encoding genes
虽然同源性较低，但酶活性表达较强，认为该基因是编码内切葡聚糖酶的一个新基因片段。 |
| 2. | This article dealt with cloning and sequencing of chitinase and endoglucanase genes of bacillus spp . and recombinant biocontrol isolates of bacillus spp
质粒分析证明克隆子中含有重组质粒，外源插入片段大小为2 . 6kb左右，与pcr最初产物的大小一致。 |
| 3. | Sequencing analysis showed that the sequence of endoglucanase fragment exhibits 35 % homology with b . subtil is chromosomal dna ( from glyb to apre ) , and 27 % homology with bacillus sp
将此重组质粒phchi转化巨大芽抱杆菌b . megateriumap25 ，得到两株转化子，分别命名为p25113一9 ， p25113一10 。 |
| 4. | By transformation with the genes . plant disease biocontrol bacteria bacillus subtil is aplls and b . megaterium ap25 were isolated from wheat field soils collected from south australia and tai an . enzyme activity analysis on chitin agar and abp media showed that b . subtilis aplls secreted chitinase and b . megaterium ap25 secreted endoglucanase , respectively
测序后在genebank上进行序列比较，该基因片段同编号为2634966的枯草芽孢杆菌全序列的2599451到2812870 (功能未知)有85的同源性，但同已发表的13种几丁质酶的基因(包括枯草芽孢杆菌几丁质酶基因)的同源性很低，只有30 。 |
| 5. | Bp23 celb genes , b . pwnilus endoglucanase and b . polymyxa beta - 1 , 4 - endoglucanase " genes , respectively . it was recognized as a new gene encoding for endoglucanase of b . mega terium . the recombinant plasmid tvchi ( pmd18 ~ t inserted with chitinase encoding gene from aplls ) and e . coli - bacillus shuttle vector physooplk were digested by ecori and sail completely , and the chitinase gene was ligated with shuttle vector , and the recombinant vector was used to transform b . megaterium ap25 competent cell
平板拮抗实验同野生菌株相比，转化子对麦长蠕抱菌的抑制作用最明显，抑制百分数最高可达33 . 3 % ，而apll3和ap25分别是23 . 1 %和25 . 6 % ，同时转化子对小麦纹枯病菌、棉花立枯病菌、棉花枯萎病菌和小麦的全蚀病菌也具有较为明显的抑制作用。 |
| 6. | 3 . 2 . 1 . 4 ) which was prepared by precipitation of the water extract of the culture of aspergillus niger with ammonium sulfate and desalted by sephadex g - 25 , and was further fractionated by two steps of deae - toyopearl 650m and one step of poros 20pi chromatography . the other was a p - glucosidase ( ec . 3 . 2 . 1 . 21 ) which was prepared by the above g - 25 fractions and was further fractionated by two steps of deae - toyopearl 650m chromatography . the specific activity of the endoglucanase with sodium carboxymethyl cellulose was estimated to be 433 . 38 hj / mg
-葡萄糖苷酶对水杨素的比活力为597 . 12iu mg ，并对其专一，不能水解棉花和羧甲基纤维素钠；分子量为117 . 5kda ，加dtt后分子量不变；该组分最适ph和温度分别为4 . 5和70 ，在ph5 . 0 、 50下对水杨素钠的米氏常数km为3 . 73mg ml ，最大反应速度vm为0 . 088mg葡萄糖( ml ? min ) ；与文献中从黑曲霉中分离的-葡萄糖苷酶比较后发现，该组分是一个新的-葡萄糖苷酶。 |
| 7. | Its km for sacilin at50 andph5 . owas3 . 73mg / ml . with the analysis of kinetics , the p - glucosidase showed synergistic action to the endoglucanase and the endoglucanase inhibited the p - glucosidase . the mechanism for their interaction was explained that the endoglucanase was combined with its products and the inhibition of these products to endoglucanase was removed by its hydrolysis by p - glucosidase
经两组分共同作用的酶反应动力学分析后发现，两组分这种相互作用机制的核心是：内切酶和?葡萄糖苷酶与内切酶的水解产物，也是其底物类似物? ?纤维二糖和纤维寡糖的结合以及-葡萄糖苷酶对该产物的水解。 |
