The insulin - like growth factor - 2 ( igf - 2 ) and igf conjugated protein - 6 ( igfbp - 6 ) mrna level in rat calvaria bone tissue and mc - 3t3 - el cells were detected by northern blotting analyses and reverse transcription polymerase chain reaction . the estrogen responsive element ( ere ) in igfbp - 6 gene promoter was identified and involved in tcdd - reduced regulation of the gene expression by electromobility shift assays ( emsa ) 正常胎鼠头盖骨组织igf一2mrna呈高水平表达状态，而igfbp一6mrna的水平较低; ccf胎鼠头盖骨骨组织内igf一2mrna的表达较正常胎鼠降低， igfbp一6mrna的表达则明显升高; atra和e :联合应用时， atra可以抑制雌激素对细胞内igf一2和igfbp一6的这种调节作用。
