| 1. | High - property chitosan can be produced by chitin deacetylase
而采用甲壳素脱乙酰酶能制备出性能独特的壳聚糖。 |
| 2. | In this paper , advances in the application and research of chitin deacetylase in preparation of chitosan are summarized
本文就甲壳素脱乙酰酶在壳聚糖制备中的应用及研究进展作一综述。 |
| 3. | 5 , histone acetyltransferase gene ygcns and histone deacetylase gene yrpd3 were cloned and expressed , which has laid down a foundation for studying chromosome remodeling in vitro
5 ，我们克隆并纯化了酵母中的组蛋白乙酞基转移酶基因gcns和去乙酞化酶基因rpd3 。 |
| 4. | 4 , compared the influence of two histone deacetylase inhibitors on the hsp70 gene transcription , after changing the time and the concentration of the inhibitors , we found the mechanism of the two histone deaceylase inhibitors is different
4 ，通过比较改变喂食时间和喂食浓度后两种去乙酞化酶抑制剂与热休克基因表达水平之间的关系，我们发现tsa与丁酸盐抑制去乙酞化酶的作用机制可能是不同的。 |
| 5. | 2 , the hsp gene transcription was quantitatively determined by rt - pcr . based on this result , it is concluded that the change of acetylation level at the loci of hsp , mediated by histone deacetylase inhibitors , exerts important functions in hsp gene transcription . 3 , after immunolabeling with anti acelated - lysine monoclonal antibody on the polytene chromosomes of heat shocked flies , fluorescence signals were detected at the hsp loci
2 ，利用rt - pcr技术，对去乙酰化酶抑制剂处理后的果蝇的热休克基因的表达水平进行检测，结果表明经组蛋白去乙酰化酶抑制剂处理后的果蝇幼虫，其热休克基因的表达高于基础水平，也就是说去乙酰化酶抑制剂诱导了热休克基因的表达。 |
| 6. | 1 , the histone deacetylase inhibitors were used to feed the larve of the fly , and then the polytene chromosomes were observed under the microscope . the results indicated that the histone deacetylase inhibitors had effects on the morphology of the polytene chromosomes . it is suggested that the acetytion has effects not only on the molecular interaction but also on the structure of the chromosomes
得到的主要结果和结论如下： 1 ，通过用去乙酰化酶抑制剂处理果蝇幼虫，观察果蝇多线染色体在热休克基因处的形态变化，发现去乙酰化酶抑制剂介导的乙酰化水平的升高可引起染色体结构的显著变化，推测乙酰化修饰不仅影响分子间的相互作用而且还可以影响染色体的高级结构。 |
| 7. | Acetylornithine deacetylase is the key enzyme of producting l - methionine . we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase . in order to get high expression deacetylase strain , we obtain the gene by pcr arge gene . the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr . then taking the nucleotide sequencing compared with the sequence at blast of u . s . a . we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g
为了获得高效表达的脱乙酰鸟氨酸酶工程菌株，在工程菌技术改造及其固定化研究做了进一步的研究和探讨。我们采用基因工程技术，通过pcr技术扩增出了酰化酶关键酶基因?脱乙酰鸟氨酸酶基因arge ，将其克隆到puc19载体中，经酶切鉴定、 pcr鉴定筛选出重组阳性质粒，并测序鉴定，通过美国blast程序进行了基因数据库相似性比较分析。 |
| 8. | 6 , we used gcn5 and rpd3 genes as probes to detect the homologous sequences in drosophila melanogaster by fluorescence in situ hybridization ( fish ) . this work has provided useful information for the localization and cloning of related histone acetyltransferase and histone deacetylase genes in drosophila melanogaster
6 ，利用己获得的酵母gcns和rpd3基因为探针，对果蝇多线染色体进行原位杂交实验，试图找出与gcns和rpd3基因同源的基因片段。为今后克隆和分离果蝇中与乙酞化和去乙酚化相关的基因奠定基础。 |
| 9. | At mean time , we have studied on the characteristic of proteinase and make sure the suitable condition and influencing factor of deacetylase function . usin g the technology of immobiolized cell , the utlization of deacetylase have been increased deeply and lower production cost . the half deline period is more than 20 days and the production of l - methionine is above 75 %
采用固定化细胞技术，使酶的利用率得以大幅度提高，固定化细胞酶活的半衰期达20天以上，可连续拆分使用生产24天， l - met产率可达75以上。 |
| 10. | By treating the cells in s , g2 phase and prophase with histone deacetylase inhibitor tsa , and through the application of microscopic observation and western - blotting , we demonstrated that histone acetylation modification played important roles in the cell cycle regulation in physarum polycephalum , affecting the normal crossover of the checkpoints of s / g2 , g2 / m and mitosis exit
这些炎白的表达水平具有细胞周期依赖性，随着细胞周期的进行而发生变化。 tsa处理引起的多头绒泡菌s期、 gz期和前期细胞内组蛋白h3乙酚化水平的提高，改变了细胞内类cyclinbi蛋白、类。 |
