In previous study , janghong have succeeded cloning 24 ests of mouse testis spermatogenic cell apoptosis - related gene by creating mouse cryptorchidism model and making use of suppression subtractive hybridization , and registered in genbank 在前期的研究工作中，本室姜宏等通过建立小鼠隐睾模型，运用抑制消减杂交法( suppressionsubtractivehybridization ， ssh )克隆出24个小鼠睾丸生精细胞凋亡相关基因的est ，并在genbank中登录。
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A single dose of des caused a significant decrease in l - pgds expression , whereas testosterone propionate supplementation could restore l - pgds expression in the eds - treated rats . after unilateral cryptorchidism , l - pgds expression in rat abdominal testis and epididymis was significantly reduced as a time - dependent fashion from 5 days after operation Eds处理后， l - pgdsmrna和蛋白在大鼠睾丸和附睾各段上皮中的表达明显降低，而睾酮处理可部分恢复l - pgds在睾丸和附睾的表达。
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Part 1 : identification of a novel gene , tsarg2 , and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell , and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis . to rapidly attain human novel gene full - length cdna sequence , the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe , which was significantly changed in cryptorchidism and represents a novel gene . furthermore , a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line 本研究分为三个部分，其主要实验方法及实验结果如下：第一章tsarg2基因的克隆与序列分析从已获得的在隐睾和正常睾丸对照中表达量有明显差异的est片段( be644542 )入手，设计了基因特异性引物和载体特异性引物进行巢式pcr扩增，结合人类基因组草图搜索法，从睾丸cdna文库中快速分离出人类睾丸凋亡相关基因的5末端而获得全长cdna ， genbank登录号为ay040204 ，同时应用生物信息学的方法克隆了该基因在小鼠中的同源基因， genbank登录号为af395083 。
