After breast feeding , their descendents were fed with the same water , then killed them on the 1st , 8th , 15th , 22nd , 30th day after birth respectively and took out of the brains , stored at - 70 . ( 2 ) measurement of the activity of pkc in brains : in cytoplasma ; took out the samples , cut the hippocampi into pieces and added buffer ( glucose 0 . 32mol / l , hepes ph 7 . 5 ) , then shattered with ultrasound , centri - fuged at 27000g for ih at 4ti , moved the supernant into a new tube 本研究以细胞信号转导途径为线索，分别探讨了pkc 、 erk 、 nos1等信号分子及即早基因( c - fos ， c - jun )在慢性铅暴露小鼠脑细胞中的活性、蛋白表达及mrna表达的改变，以及铅暴露小鼠记忆行为的变化，并将细胞中信号分子的改变与记忆功能改变结合进行分析以寻找一些内在规律。
