| 1. | The cell proliferation was observed with flow cytometer and mtt colorimetric test
Atra明显拮抗了雌激素对细胞增殖的正性调节作用。 |
| 2. | The numbers of differentiated cells induced by bmp - 2 , atra , vd3 and dex were not correlative with the amount of ra receptor mrna
Bmp一2 、 atra 、 vd3和dex诱导形成的分化细胞量与ra受体mrna表达量之间不存在相关性。 |
| 3. | The differentiation effects of neural stem cells treated by bmp - 2 , atra , vd3 and dex are not correlative with the expression of ra receptor mrna
Bmp一2 、 afra 、 vd3和dex对神经干细胞分化的诱导作用与ra受体mrna表达量的改变没有相关性 |
| 4. | Bfgf is essential for the proliferation and differentiation of neural stem cells , it can also affect the process of expression of rara mrna induced by atra or bmp - 2 ; 2
Bfgf在神经干细胞增殖与分化中都起着必不可少的营养作用， bfgf能影响al ， ra 、 bmp一2对raramrna表达的诱导 |
| 5. | Neural stem cells can be induced to differentiate into neuronic cells by atra , vd3 and dex , and into astrocytes by bmp - 2 ; atra and bmp - 2 antagonize each other . 3
神经干细胞能被atra 、 vd3和dex诱导形成神经元样细胞，被bmp一2诱导形成星形胶质细胞， atra与bmp一2作用相互拮抗 |
| 6. | In the later experiments dmem - lg / 20 % fbs / bfgf ( 50ng / ml ) was used as the neuronal preinduction media and dmem - lg / atra ( 150mg / ml ) was used as the neuronal induction media
在以后的实验中， bfgf瞩0n才rnl ）被用来作为前诱导剂，人ttiho50略inl ）被用来作为诱导剂。 |
| 7. | Conclusions : these data may proide a rationale to explore clinical use of atra for the preention of radiation - induced lung fibrosis and other pathologic conditions inoling pulmonary fibrosis
结论：本研究资料为探索全反式维甲酸预防辐射和其他病理条件诱发肺纤维化的临床应用提供了理论依据。 |
| 8. | Over the induction process cell body became increasing spherical and retractile , exhibiting a typical neuronal perikaryal appearance . western blot a - nalysis indicated that cells exhibited increased expression of the neuronal marker nse after 5h of bfgf , atra treatment
免疫细胞化学显示在加人bme5小时后部分细胞胞体收缩变圆，胞质呈nse染色强阳性，细胞呈有较长突起的双极，多极型。 |
| 9. | The ere oligonucleotides were chemically synthesized , annealed , and labeled at the 5 " - ends by polynucleotide kinase and r - 32p atp ( 3000 ci / mmol ) . for competed experiment the 400 fold molar excess of the cold ere oligonucleotides were added to reaction mixture
结论: 100一140m盯kg浓度的atra可以诱导大鼠骨骼畸形发育并产生ccf ; igfs家族基因中igf一2和igfbp一6表达的调控与大鼠骨骼发育以及成骨细胞的增殖密切相关。 |
| 10. | Dab served as chromagen . western blot thirty micrograms of protein extracted from untreated and bfgf , atra - induced mmscs cultures were separated on a 8 % gradient acrylamide gel and eletrophoretically transferred to a nitrocellulose membrane . the blot was probed for nse expression
4westernblot检测诱导后细胞的nse表达情况从未经处理和经过bfgf ， atra诱导的细胞中提取30爬蛋白在8的sds一聚丙烯酸胺凝胶上电泳并转移到硝酸纤维素膜上， 4 5脱脂奶粉封闭过夜。 |
