Application of mri score in diagnosis of astrocytic tumors 评分在星形细胞起源肿瘤诊断中的应用
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The velocity of astrocytic intracellular calcium elevation to the peak value is a slightly faster than neurons Ast钙水平达到峰值的速度略快于神经元。
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Objective : in this study we try to simultaneously investigate the response of neurons and astrocytes of rats following hyperosmotic stimulation and focus attention on the role of neuronal - astrocytic complex in the osmotic reglution 目的:本研究试图从整体水平共同观察神经元和ast对高渗透压刺激的反应,探讨神经胶质细胞?神经元复合体在渗透压调控过程中的作用。
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2 , the electron dense area ( eda ) consisting of the astrocytic process on one side and the neuron ( dendrite ) on the other side was observed in immune - electron - microscopic staining studies , and the eda was characterized with double layers thickening and dark staining cytomembranes with a narrow cleft between them ( 2 )免疫电镜观察, son内星形胶质细胞与神经元接触部位可以观察到膜增厚的结构? ?电子致密区( edas ) ,在神经元一侧可见cx32阳性金颗粒,而在星形胶质细胞一侧可见cx43阳性物质分布。
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Fos + / th + / gfap + and fos + / vp + / gfap + triple labeled n - asc could be found in the mvz , pvn and son respectively ; ( 2 ) under electronic microscope , the astrocytic processes connected closely with the dendrites or axons of the neurons , where the bilateral membranes became thick . we call transiently it electron - dense areas ( edas ) . the number of edas increased remarkably following hyperosmotic stimulation ; ( 3 ) when trace retrogradely , wga - hrp was microinjected into the unilateral son , pvn or nucleus of solitary tract ( nts ) respectively using the stereotaxic method , the n - ascs formed by the neurons triple - labeled with hrp / fos / th ( or vp ) and astrocytes labeled with gfap could be found in the mvz , son and pvn respectively ; ( 4 ) after being treated with heperosmotic nacl solution , intracellular calcium concentration in cultured hypothamic neurons and astrocytes increased and then decreased 脑内gfap阳性结构也明显增多,其分布与fos阳性细胞分布基本一致,表现为胞体肥大、突起粗长; ast紧密包绕在神经元周围形成神经元- ast复合体( n - asc ) ;在mvz 、 pvn和son三重免疫组化染色切片上可见到fos + th + gfap +第四军医大学博士学位论文和fos vp gfap三重标记asc ; ( 2 )免疫电镜下son内星型胶质细胞突起与神经元树突或轴突之间接触部位出现增厚的膜结构一电于致密区( edas ) ,高渗刺激后数量明显增多: ( 3 )将们个mp注入大鼠一侧n卜、卜卜或孤束核( ws ) ,分别在延髓内脏带( mvz ) 、 so和pvn内出现fos hrp th 、 fos hrp八p三重标记神经元和gfap阳性标记ast形成的n asc ; ( 4 )高渗刺激使培养神经元和ast内钙水平先升高后降低,最后维持在比高渗刺激前稍高的静息钙水平上。