Absorption , fluorescence emission and excitation spectra of five pigment - protein complexes were determined . photosynthetic electron transfer was measured from the dcip photoreduction . p700 concentration was assayed from the ferricyanide - oxdised minus ascorbate - reduced difference spectrum 测定裙带菜各色素蛋白复合物的吸收光谱、荧光发射光谱和荧光激发光谱，并进行了dcip的光还原测定和化学法的氧化还原差示光谱测定。
4.
According to the research of physiological and biochemical indicators or index , components of soluble proteins , substrate protein of phosphorylation and the activity of protein kinase in low - temperature stress in the leaves of brassica oleracea l . , we tried to find the law of the physiological and biochemical response of brassica oleracea l . leaf to low temperature . at the same time , discussion on the signal transduction can also provide further evidences for revealing the mechanism of low - temperature stress . the results are showed as follows : malondialdehyde ( mda ) , superoxide dismutase ( sod ) , ascorbate peroxidase ( asp ) and peroxidase ( pod ) activities were changed greatly after 0 ~ 30min ' s treating with low temperature 本文以甘蓝叶片为材料，通过对低温5胁迫下甘蓝生理生化指标、可溶性蛋白组分以及磷酸化底物蛋白、蛋白激酶活性的研究，以期找出甘蓝叶片对低温胁迫的生理生化响应规律，为甘蓝露地越冬栽培防范寒害提供理论指导，同时对低温胁迫下甘蓝逆境信号传导进行了探讨，从而为彻底弄清低温胁迫机理提供进一步的证据，研究的主要结果如下：丙二醛含量( mda ) 、超氧化物歧化酶( sod ) 、抗坏血酸过氧化物酶( asp )和过氧化物酶( pod )活性在低温处理0 30min发生显著变化，低温处理3min后，甘蓝叶片内mda含量基本没有变化，处理5min时出现第一个峰值，达到对照的104 . 10 ， 10min出现低谷，仅为对照的86 . 27 ，随后再次上升， 30min时超过第一峰值，为对照的113 . 93 。
5.
In this experiment , seedlings of arabidopsis thaliana ( col ) were observed after being treated by verlicillium dahliae ( vd - toxin ) , exogenous salicylic acid ( sa ) , nitric oxide donor ( snp ) and nitric oxide synthase inhibitor ( nna ) , then we investigated the changes of endogenous h2o2 content , the activity of the antioxidant enzymes catalase ( cat , ec : 1 . 11 . 1 . 6 ) and ascorbate peroxidase ( apx , ec : 1 . 11 . 1 . 11 ) and mrna levels of cat3 in different stress conditions , we also identified the localizations of h2o2 and no accumulated in the leaves of arabidopsis 本实验研究了棉花黄萎病菌?大丽轮枝菌毒素( vd - toxin )与拟南芥幼苗互作反应中外源sa 、 no供体snp 、 no合酶抑制剂nna等不同处理对拟南芥幼苗h _ 2o _ 2含量、 cat和apx活性及cat基因mrna表达量的影响，并对no 、 h _ 2o _ 2的积累部位进行染色检测。
