English translation for "体外转录"

in vitro transcription
transcription in vitro

Related Translations:

转录间歇:  transcription pause

转录过程:  transcription

转录机:  re recordertranscription machinery

转录抑制:  transcription inhibition

转录子:  transcripton

对称转录:  symmetric transcription

病毒转录:  viral transcription

转录设备:  transcription device

转录程序:  transducer

转录变异:  transcriptional variation

Example Sentences:

	1.	Linearized full - length cdna was used as template then genomic rna of csfv was in vitro transcriped by t7 rna polymerase 以线性化的全长cdna为模板，体外转录得到了csfv基因组rna 。
	2.	The ligation products were transfected to jm109 competent cells . the positive clones were confirmed by pcr , then the positive plasmids were minipreped , digested and sequenced 线性化质粒，分别用竹和sp6rna多聚酶，掺人3 ， s一dutp ，体外转录正义和反义探针。
	3.	Different migs / substrate reacting concentrations were tested , the best migs / substrate ratio was 2 : 1 and too much m1gs led to substrate degrading . it was also found that the secondary structure affected the action of m1gs 对m1gsdna模板和4个底物模板分别进行体外转录，获得核酶m1gs的rna片段以及带有~ ( 32 ) p放射性标记的四个底物rna片段。
	4.	We tested the interaction of these two proteins using in vitro transcription and translation system and coimmunoprecipitation . it was shown that the two proteins could interact in vitro and the interaction was ca ~ ( 2 + ) dependent 利用体外转录翻译系统和免疫共沉淀验证了这两种蛋白在体外的相互作用，结果表明ked样蛋白能够在体外与acamz发生相互作用，并且这种作用是c扩+依赖型的。
	5.	At first , the vrl - cdna plasmids were transformed into tg1 r . coli to enlarge vrl - cdna ; then the plasmids were extracted and cut off with enzymes ; subsequently , vrl - cdnas were transcribed into vrl - inrna by t7 or sp6 polymerase in vitro , following vrl - mrnas were injected into xenopus laevis oocyte to express into vr1 receptor protein ; at the end the vrl + - oocytes were tested by double electode voltage clamp 这项实验中，我们首先将大白鼠vr1 - cdna质粒转入大肠杆菌中进行扩增，然后提取质粒并酶切，然后在体外转录成vr1 - mrna ，接着将vr1 - mrna注射到非洲爪蟾卵母细胞中表达为蛋白质受体，建立了实验模型细胞，最后用双微电极电压钳检测此模型细胞。
	6.	1 . expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes , an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro . the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers 将cry基因的高保守区的cry a ( a ) ecog - f片段插入带有t7rna聚合酶启动子的质粒pselect - 1 ，获得了能在体外转录的rna探针载体pbpl - 1 ，用该载体制备的rna探针具有特异强，背景清楚，省时省力等优点，已成功地用于苏云金芽胞杆菌的分子生物学研究和特异菌株的筛选。

Similar Words：

"体外震波碎石术" English translation, "体外治疗室" English translation, "体外肿瘤性转形" English translation, "体外重组" English translation, "体外转化灶分析" English translation, "体外转录,无细胞转录" English translation, "体外转录法" English translation, "体外转录分析" English translation, "体外自动除颤器" English translation, "体外自动电击器" English translation