体外转录 meaning in English
in vitro transcription
transcription in vitro
Examples
- Linearized full - length cdna was used as template then genomic rna of csfv was in vitro transcriped by t7 rna polymerase
以线性化的全长cdna为模板，体外转录得到了csfv基因组rna 。 - The ligation products were transfected to jm109 competent cells . the positive clones were confirmed by pcr , then the positive plasmids were minipreped , digested and sequenced
线性化质粒，分别用竹和sp6rna多聚酶，掺人3 ， s一dutp ，体外转录正义和反义探针。 - Different migs / substrate reacting concentrations were tested , the best migs / substrate ratio was 2 : 1 and too much m1gs led to substrate degrading . it was also found that the secondary structure affected the action of m1gs
对m1gsdna模板和4个底物模板分别进行体外转录，获得核酶m1gs的rna片段以及带有~ ( 32 ) p放射性标记的四个底物rna片段。 - We tested the interaction of these two proteins using in vitro transcription and translation system and coimmunoprecipitation . it was shown that the two proteins could interact in vitro and the interaction was ca ~ ( 2 + ) dependent
利用体外转录翻译系统和免疫共沉淀验证了这两种蛋白在体外的相互作用，结果表明ked样蛋白能够在体外与acamz发生相互作用，并且这种作用是c扩+依赖型的。 - At first , the vrl - cdna plasmids were transformed into tg1 r . coli to enlarge vrl - cdna ; then the plasmids were extracted and cut off with enzymes ; subsequently , vrl - cdnas were transcribed into vrl - inrna by t7 or sp6 polymerase in vitro , following vrl - mrnas were injected into xenopus laevis oocyte to express into vr1 receptor protein ; at the end the vrl + - oocytes were tested by double electode voltage clamp
这项实验中，我们首先将大白鼠vr1 - cdna质粒转入大肠杆菌中进行扩增，然后提取质粒并酶切，然后在体外转录成vr1 - mrna ，接着将vr1 - mrna注射到非洲爪蟾卵母细胞中表达为蛋白质受体，建立了实验模型细胞，最后用双微电极电压钳检测此模型细胞。