转录起始位点 meaning in English
transcription initiation site
Examples
- Computational location of transcription start sites in prokaryotic genome based on sliding window
基于滑动窗口的原核转录起始位点计算定位方法 - By artificially changing a to c at - 137 bp site upstream from transcription start point of cloned promoter , two site - mutation promoters , ipms ( 603 bp ) and ipm1 ( 900 bp ) were created
通过pcr方法对克隆的两个启动子进行定点突变,使转录起始位点上游- 137bp处a突变为c ,得到两个突变启动子( ipml 、 ipms ) 。 - Activity of each construct was determined . the basal promoter was located at about 60bp up stream of the transcription initiation site . it contains a tata box at - 33bp which is required for the transcription initiation
基因的基本启动子元件位于转录起始位点上游约60bp ,其中含有一个位于- 33bp处的tatabox ,它对于转录起始起着重要作用。 - 900 bp promoter - directed gus expression was highly induced by sa and bth , while the 603 bp promoter , whether mutated or not , did not respond to sa and bth induction , which indicated that the element in response to sa and bth lied among 575 ~ 872 bp from transcription start site
全长900bp启动子能够应答sa和bth的诱导,而603bp长的启动子无论突变与否对sa和bth均无应答,证明sa和bth的应答区域在克隆启动子的转录起始位点上游575 872bp之间。 - In order to use the responding ability to the inducers of pr - la promoter , two fragments , ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank . sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence . its transcription start site , tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter
根据pr - 1a基因的报道序列,设计两对引物,以烟草基因组为模板,通过pcr扩增得到900bp ( ipl )和603bp ( ips )两个目标片段,序列测定表明克隆的启动子与报道序列具有99的同源性,转录起始位点、 tatabox及保守序列tgac与报道序列均完全相同。