克隆片段 meaning in English
cloned sequence
Examples
- Plasmid pucis was used to construct viral genome library . ten fragments were obtained and some of them were partially sequenced and analysed using genbank data
利用puc18质粒构建了织线藻病毒部分基因组的dna文库，获得了10个基因组片段，作了部分克隆片段的序列测序和分析。 - After selecting kanamycin resistant transformants on ms medium , their dna were extracted for pcr and dot blot . in pcr , the 1 . 488kb fragment was amplified , and the result of dot blot was positive
生长曲线表明，带有克隆片段的转化株在0 . 5mol lnacl的高渗透压培养基中生长良好，而作为对照的缺失株则几乎不能生长。 - The experimental procedure that begins with a cloned segment of dna , or a protein sequence , and uses this knowledge to introduce programmed mutations ( through directed mutagenesis ) back into the genome in order to investigate gene and protein function
在已知dna克隆片段或蛋白质序列上引导程序性的变异（通过直接诱变）并返回到基因组，来研究基因和蛋白质功能的实验方法。 - Phaa , phab and phac were amplifed from the subclone of pseudomanas sp . producing pha by pcr . the gel electrophoresis analysis showed that the molecular weights of cloned phaa , phab and phac were equal to fragment speculated from three orfs
利用所提取的开放阅读框架的序列设计三对引物，采用pcr技术，从合成pha的亚克隆片段中分离出phaa 、 phab和phac三个基因片段，经凝胶电泳分析表明，所克隆的三个基因分子量大小与推测的三个开放阅读框架中基因片段大小一致。 - The cloning cdna fragment was extracted from positive clones and sequenced . the results showed that the cdna fragment was 816bp in size , encoding a protein which included 272 amino acids . the sequence homology analysis was carried out via the software blast 2 . 0 network service in the four large databases - genbank , embl , ddbj , pdb , which had recorded 1 337 978 nucleotide and protein sequences . the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango , passion fruit , persia plum , strawberry , grape . . . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 % . from the results mentioned above , we could confirm that the cdna of rubber tree etrl had been cloned
从阳性克隆子中提取克隆片段，经序列测定分析，结果表明，克隆片段的cdna大小为816bp ，编码的蛋白质包含272个氨基酸。基因序列通过blast2 . 0networkservice软件对genbank ， embl ， ddbj ， pdb四个大型数据库中记录的1337978条核酸和蛋白质序列进行序列相似性检索，结果表明与芒果、一西番莲、波斯梅、草毒、葡萄、西洋梨等15种已报道的植物的etrl基因cdnag的同源率为75 88 ；蛋白质氨基酸序列的同源率为90 95 ，表明本研究确实克隆到了橡胶树etri基因的cdna序列。 4